{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:08:06Z","timestamp":1788923286930,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Donor scarcity limits islet transplantation for type 1 diabetes. Early postnatal piglets are a potential xenogeneic source of endocrine tissue, yet their immature islet cell clusters (ICCs) remain insufficiently characterised with respect to size and maturation. Here, we quantified ICC size heterogeneity in pancreata from six 14\u2010day\u2010old piglets using insulin and glucagon immunofluorescence staining and large\u2010area immunofluorescence imaging with automated whole\u2010section analysis. ICCs were classified as small (1\u20132 endocrine cells), intermediate (3\u20135 endocrine cells), or large (\u22656 endocrine cells). Well\u2010developed ICCs reaching \u223c100\u00a0\u00b5m in diameter were consistently identified in all animals and exhibited an intermingled \u03b2\u2010 and \u03b1\u2010cell cytoarchitecture similar to that of adult pig and human islets. The densities of small, intermediate, and large clusters were largely comparable among littermates and across piglets, suggesting a consistent distribution of ICC size classes at this postnatal age. Quantitative composition analysis revealed a strong predominance of insulin\u2010positive area. In small and intermediate ICCs, we observed mean \u03b2\/\u03b1 cell ratios of 3.7\u00a0\u00b1\u00a00.4 and 3.8\u00a0\u00b1\u00a00.4, respectively. In large ICCs, the proportion of glucagon staining was significantly higher, decreasing the \u03b2\/\u03b1 cell ratio to 2.2\u00a0\u00b1\u00a00.3. Together, these data point to a reproducible distribution of ICC sizes in 14\u2010day\u2010old piglet pancreata, including a population of morphologically well\u2010developed, \u223c100\u00a0\u00b5m\u2010sized clusters. These findings from native pancreatic tissue provide a morphological basis for further evaluating 14\u2010day\u2010old piglets as a potential donor source for xenogeneic islet transplantation.<\/jats:p>","DOI":"10.1111\/xen.70164","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:51:05Z","timestamp":1788922265000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantification of Islet Cell Cluster Size Heterogeneity in Early Postnatal Porcine Pancreas"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9744-268X","authenticated-orcid":false,"given":"Daviana Munoz","family":"Cruz","sequence":"first","affiliation":[{"name":"Surgical Research Unit, Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Frick","sequence":"additional","affiliation":[{"name":"Surgical Research Unit, Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Adamina","sequence":"additional","affiliation":[{"name":"Surgical Research Unit, Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9o","family":"B\u00fchler","sequence":"additional","affiliation":[{"name":"Surgical Research Unit, Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3789-6464","authenticated-orcid":false,"given":"Carmen","family":"Gonelle\u2010Gispert","sequence":"additional","affiliation":[{"name":"Surgical Research Unit, Faculty of Science and Medicine University of Fribourg  Fribourg Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1366530"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002354"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368917X694859"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2506549"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X662372"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0216254"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1032906"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118649"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12184"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3089.1999.00009.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3727\/000000003783985142"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12793"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12130"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12666"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510790103"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110384"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2022.101595"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06\u20101307"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.55.03.06.db05\u20100839"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38008"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.70039"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047686\u20107"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/TXD.0000000000000599"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70164","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/xen.70164","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:51:07Z","timestamp":1788922267000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/xen.70164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/xen.70164"],"URL":"https:\/\/doi.org\/10.1111\/xen.70164","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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