{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:50:11Z","timestamp":1792054211132,"version":"4.4.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":44,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cellular xenotransplantation offers a unique immunotherapeutic strategy for cancer by utilizing xenogeneic cells to elicit robust host immune responses. Innovative treatments are required for non\u2010muscle invasive bladder cancer (NMIBC) due to the limited effectiveness of current standard therapies. Intravesical xenogeneic porcine urothelial cell (PUC) immunotherapy provides a novel mechanism\u2010of\u2010action option.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study evaluates cellular xenotransplantation utilizing primary PUCs in vitro and in vivo to characterize their potential as an intravesical immunotherapeutic agent for NMIBC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Primary PUC phenotypic and transcriptomic identities were first characterized using flow cytometry and bulk RNA sequencing. Subsequently, the effects of basic fibroblast growth factor (bFGF) and the ROCK inhibitor Y\u201027632 on PUC expansion capacity, gene expression, and senescence were investigated. Immune\u2010stimulatory potency was measured through cell\u2010based co\u2010culture bioassays, and in vivo biodistribution and immune recruitment were assessed in mice following intravesical instillation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Expanded PUCs demonstrated high epithelial purity (92.16% \u00b1 5.38% CD49f+). RNA\u2010seq profiles confirmed a distinct urothelial identity (e.g., UPK3A, TP63, KRT7), with unsupervised clustering clearly separating PUCs from skeletal muscle comparators. Combined treatment with bFGF and Y\u201027632 synergistically promoted growth and upregulated differentiation markers while significantly suppressing p16\u2010mediated senescence. PUCs significantly stimulated immune cell proliferation and IFN\u2010\u03b3\/IL\u20102 secretion through direct, F\u2010actin\u2010polarized interactions. in vivo, PUCs remained localized and were cleared within 28 days, inducing a peak recruitment of CD4+ and CD8+ T cells at Day 14 and enhancing systemic cytotoxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PUCs serve as a potent, localized, and transient cellular medicine that primes systemic antitumor\u2010like immunity. The optimized expansion protocol facilitates the scalable production of non\u2010senescent porcine cells for clinical applications in bladder cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/xen.70167","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:35:57Z","timestamp":1792053357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro and In Vivo Characterization of Porcine Urothelial Cells as an Intravesical Cellular Xenotransplantation Immunotherapeutic Agent for Bladder Cancer"],"prefix":"10.1111","volume":"33","author":[{"given":"Chih\u2010Rong","family":"Shyr","sequence":"first","affiliation":[{"name":"eXCELL Biotherapeutics INC  Taichung Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen\u2010Kai","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Medicine Urology Division China Medical University and Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Feng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medicine Urology Division China Medical University and Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7116-4304","authenticated-orcid":false,"given":"Wen\u2010Lung","family":"Ma","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences, and Ph.D. Program for Health Science and Industry, School of Medicine China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4762-8877","authenticated-orcid":false,"given":"Chi\u2010Ping","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Medicine Urology Division China Medical University and Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1177\/09636897211011995","article-title":"Utilizing Xenogeneic Cells as a Therapeutic Agent for Treating Diseases","volume":"30","author":"Huang C. P.","year":"2021","journal-title":"Cell Transplantation"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12935-018-0501-7","article-title":"Xenogeneic Cell Therapy Provides a Novel Potential Therapeutic Option for Cancers by Restoring Tissue Function, Repairing Cancer Wound and Reviving Anti\u2010Tumor Immune Responses","volume":"18","author":"Huang C. P.","year":"2018","journal-title":"Cancer Cell International"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.ijsu.2015.07.703","article-title":"Current Status of Islet Xenotransplantation","volume":"23","author":"Park C. G.","year":"2015","journal-title":"International Journal of Surgery"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1097\/MOT.0b013e3283292522","article-title":"Cellular Xenotransplantation","volume":"14","author":"Poncelet A. J.","year":"2009","journal-title":"Current Opinion in Organ Transplantation"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11111717"},{"key":"e_1_2_9_7_1","first-page":"2285","article-title":"Anti\u2010Tumor Activity of Intratumoral Xenogeneic Urothelial Cell Monotherapy or in Combination With Chemotherapy in Syngeneic Murine Models of Bladder Cancer","volume":"13","author":"Huang C. P.","year":"2023","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1007\/s00262-020-02775-6","article-title":"Combination of Novel Intravesical Xenogeneic Urothelial Cell Immunotherapy and Chemotherapy Enhances Anti\u2010Tumor Efficacy in Preclinical Murine Bladder Tumor Models","volume":"70","author":"Huang C. P.","year":"2021","journal-title":"Cancer Immunology, Immunotherapy"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17152448"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1177\/0963689718822773","article-title":"Intravesical Administration of Xenogeneic Porcine Urothelial Cells Attenuates Cyclophosphamide\u2010Induced Cystitis in Mice","volume":"28","author":"Huang C. P.","year":"2019","journal-title":"Cell Transplantation"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.eururo.2025.12.011","article-title":"Bladder Cancer Incidence and Mortality: A Global Overview and Recent Trends","volume":"89","author":"Filho A. M.","year":"2025","journal-title":"European Urology"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/S0140-6736(98)07422-4","article-title":"BCG Immunotherapy of Bladder Cancer: 20 Years On","volume":"353","author":"Alexandroff A. B.","year":"1999","journal-title":"Lancet"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/181987","article-title":"Bladder Cancer Immunotherapy: BCG and Beyond","volume":"2012","author":"Askeland E. J.","year":"2012","journal-title":"Advances in Urology"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"62.e1","DOI":"10.1016\/j.urolonc.2025.08.007","article-title":"Revisiting BCG: The Potential of Rescue BCG Therapy for BCG\u2010Unresponsive Non\u2010Muscle\u2010Invasive Bladder Cancer","volume":"44","author":"Bai K.","year":"2026","journal-title":"Urologic Oncology: Seminars and Original Investigations"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1200\/JCO-25-01651","article-title":"TAR\u2010200 for Bacillus Calmette\u2010Gu\u00e9rin\u2013Unresponsive High\u2010Risk Non\u2013Muscle\u2010Invasive Bladder Cancer: Results From the Phase IIb SunRISe\u20101 Study","volume":"43","author":"Daneshmand S.","year":"2025","journal-title":"Journal of Clinical Oncology"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S1470-2045(20)30540-4","article-title":"Intravesical Nadofaragene Firadenovec Gene Therapy for BCG\u2010Unresponsive Non\u2010Muscle\u2010Invasive Bladder Cancer: A Single\u2010Arm, Open\u2010Label, Repeat\u2010Dose Clinical Trial","volume":"22","author":"Boorjian S. A.","year":"2025","journal-title":"The Lancet Oncology"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/EVIDoa2200167"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1200\/JCO.2019.37.7_suppl.350","article-title":"Keynote 057: Phase II Trial of Pembrolizumab (Pembro) for Patients (Pts) With High\u2010Risk (HR) Nonmuscle Invasive Bladder Cancer (NMIBC) Unresponsive to Bacillus Calmette\u2010Gu\u00e9rin (BCG)","volume":"37","author":"Balar A. V.","year":"2019","journal-title":"Journal of Clinical Oncology"},{"key":"e_1_2_9_19_1","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.eururo.2008.03.068","article-title":"Generation of a Functional, Differentiated Porcine Urothelial Tissue in Vitro","volume":"54","author":"Turner A. M.","year":"2008","journal-title":"European Urology"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.198184"},{"key":"e_1_2_9_21_1","first-page":"583","article-title":"Normal Human Urothelial Cells in Vitro: Proliferation and Induction of Stratification","volume":"71","author":"Southgate J.","year":"1994","journal-title":"Laboratory Investigation"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.14712\/fb2005051050126","article-title":"Establishment and Characterization of Primary and Subsequent Subcultures of Normal Mouse Urothelial Cells","volume":"51","author":"Kreft M. E.","year":"2005","journal-title":"Folia Biologica"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1002\/cbin.10614","article-title":"Optimization of Porcine Urothelial Cell Cultures: Best Practices, Recommendations, and Threats","volume":"40","author":"Pokrywczynska M.","year":"2016","journal-title":"Cell Biology International"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1038\/s41597-024-04201-3","article-title":"A Chromosome\u2010Scale Reference Genome of the Banna Miniature Inbred Pig","volume":"11","author":"Chen H.\u2010M.","year":"2024","journal-title":"Scientific Data"},{"key":"e_1_2_9_25_1","doi-asserted-by":"crossref","first-page":"D886","DOI":"10.1093\/nar\/gkae1142","article-title":"CZ CELLxGENE Discover: A Single\u2010Cell Data Platform for Scalable Exploration, Analysis and Modeling of Aggregated Data","volume":"53","author":"Abdulla S.","year":"2025","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1038\/s41556-021-00788-6","article-title":"Anatomical Structures, Cell Types and Biomarkers of the Human Reference Atlas","volume":"23","author":"B\u00f6rner K.","year":"2024","journal-title":"Nature Cell Biology"},{"key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1038\/s41467-019-12307-1","article-title":"Urothelial Organoids Originating From CD49f High Mouse Stem Cells Display Notch\u2010Dependent Differentiation Capacity","volume":"10","author":"Santos C. P.","year":"2019","journal-title":"Nature Communications"},{"issue":"2","key":"e_1_2_9_28_1","doi-asserted-by":"crossref","first-page":"S70","DOI":"10.1038\/modpathol.2009.1","article-title":"Staging and Reporting of Urothelial Carcinoma of the Urinary Bladder","volume":"22","author":"Cheng L.","year":"2009","journal-title":"Modern Pathology"},{"key":"e_1_2_9_29_1","first-page":"1391","article-title":"Protein Atlas of Fibroblast Specific Protein 1 (FSP1)\/S100A4","volume":"38","author":"Fendt B. M.","year":"2023","journal-title":"Histology and Histopathology"},{"key":"e_1_2_9_30_1","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1126\/science.306682","article-title":"Acute Myelogenous Leukemia: A Human Cell Line Responsive to Colony\u2010Stimulating Activity","volume":"200","author":"Koeffler H. P.","year":"1978","journal-title":"Science"},{"key":"e_1_2_9_31_1","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1084\/jem.152.6.1709","article-title":"Biochemical and Biological Characterization of Lymphocyte Regulatory Molecules. V. Identification of an Interleukin 2\u2010producing human Leukemia T Cell Line","volume":"152","author":"Gillis S.","year":"1980","journal-title":"Journal of experimental medicine"},{"key":"e_1_2_9_32_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.70283","article-title":"Novel Insights From a Multiomics Dissection of the Hayflick Limit","volume":"11","author":"Chan M.","year":"2022","journal-title":"eLife"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.bbrc.2007.05.067","article-title":"FGF\u20102 Suppresses Cellular Senescence of Human Mesenchymal Stem Cells by Down\u2010Regulation of TGF\u2010\u03b22","volume":"359","author":"Ito T.","year":"2007","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"e_1_2_9_34_1","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.3390\/cells10113242","article-title":"The Role of Fibroblast Growth Factor (FGF) Signaling in Tissue Repair and Regeneration","volume":"10","author":"Farooq M.","year":"2021","journal-title":"Cells"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/ijc.2910430522","article-title":"Growth Stimulation of Human Breast Epithelial Cells by Basic Fibroblast Growth Factor in Serum\u2010Free Medium","volume":"43","author":"Takahashi K.","year":"1989","journal-title":"International Journal of Cancer"},{"key":"e_1_2_9_36_1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.ajpath.2011.10.036","article-title":"ROCK Inhibitor and Feeder Cells Induce the Conditional Reprogramming of Epithelial Cells","volume":"180","author":"Liu X.","year":"2012","journal-title":"American Journal of Pathology"},{"key":"e_1_2_9_37_1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.archoralbio.2015.03.003","article-title":"Y\u201027632, a ROCK Inhibitor, Delays Senescence of Putative Murine Salivary Gland Stem Cells in Culture","volume":"60","author":"Lee J.","year":"2015","journal-title":"Archives of Oral Biology"},{"key":"e_1_2_9_38_1","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/jcp.1041550218","article-title":"Expression of Basic Fibroblast Growth Factor and Its Receptor in an Invasive Bladder Carcinoma Cell Line","volume":"155","author":"Allen L. E.","year":"1993","journal-title":"Journal of Cellular Physiology"},{"key":"e_1_2_9_39_1","doi-asserted-by":"crossref","first-page":"4567","DOI":"10.1073\/pnas.1803595116","article-title":"Mouse and human Urothelial Cancer Organoids: A Tool for Bladder Cancer Research","volume":"116","author":"Mullenders J.","year":"2019","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"e_1_2_9_40_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0089834","article-title":"Clonogenicity: Holoclones and Meroclones Contain Stem Cells","volume":"9","author":"Beaver C. M.","year":"2014","journal-title":"PLoS ONE"},{"key":"e_1_2_9_41_1","doi-asserted-by":"crossref","first-page":"14016","DOI":"10.1073\/pnas.0906549106","article-title":"Identification, Molecular Characterization, Clinical Prognosis, and Therapeutic Targeting of Human Bladder Tumor\u2010Initiating Cells","volume":"106","author":"Chan K. S.","year":"2009","journal-title":"PNAS"},{"key":"e_1_2_9_42_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1309\/PYQC-17CB-063T-Q07J","article-title":"Uroplakin III Is a Highly Specific and Moderately Sensitive Immunohistochemical Marker for Primary and Metastatic Urothelial Carcinomas","volume":"113","author":"Kaufmann O.","year":"2000","journal-title":"American Journal of Clinical Pathology"},{"key":"e_1_2_9_43_1","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1006\/jsre.1995.1160","article-title":"Effect of Growth Factors on Cell Proliferation and Epithelialization in Human Skin","volume":"59","author":"Bhora F. Y.","year":"1995","journal-title":"Journal of Surgical Research"},{"key":"e_1_2_9_44_1","doi-asserted-by":"crossref","unstructured":"Y.Fujimoto T.Yokozeki A.Yokoyama andY.Tabata \u201cBasic Fibroblast Growth Factor Enhances Proliferation and Hepatocyte Growth Factor Expression of Feline Mesenchymal Stem Cells \u201d Regen Ther. 15 (2020) 10\u201317.","DOI":"10.1016\/j.reth.2020.03.013"},{"key":"e_1_2_9_45_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/sj.bjc.6606016","article-title":"Small Molecule FGF Receptor Inhibitors Block FGFR\u2010Dependent Urothelial Carcinoma Growth In Vitro and In Vivo","volume":"104","author":"Lamont F. R.","year":"2011","journal-title":"British Journal of Cancer"},{"key":"e_1_2_9_46_1","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1111\/jcmm.13222","article-title":"Rho\u2010Kinase Inhibitor Y\u201027632 Facilitates the Proliferation, Migration and Pluripotency of Human Periodontal Ligament Stem Cells","volume":"21","author":"Wang T.","year":"2017","journal-title":"Journal of Cellular and Molecular Medicine"},{"key":"e_1_2_9_47_1","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1172\/JCI42297","article-title":"Human Keratinocytes are Efficiently Immortalized by a Rho Kinase Inhibitor","volume":"120","author":"Chapman S.","year":"2010","journal-title":"Journal of Clinical Investigation"},{"key":"e_1_2_9_48_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/j.1399-3089.2004.00199.x","article-title":"History of Xenotransplantation","volume":"12","author":"Deschamps J.\u2010Y.","year":"2005","journal-title":"Xenotransplantation"},{"key":"e_1_2_9_49_1","doi-asserted-by":"crossref","first-page":"IT53","DOI":"10.1038\/80100","article-title":"Xenotransplantation","volume":"18","author":"Nature Biotechnology","year":"2000","journal-title":"Nature Biotechnology"},{"key":"e_1_2_9_50_1","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nri2099","article-title":"Xenotransplantation: Current Status and a Perspective on the Future","volume":"7","author":"Yang Y. G.","year":"2007","journal-title":"Nature Reviews Immunology"},{"key":"e_1_2_9_51_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1056\/NEJMoa2201422","article-title":"Genetically Modified Porcine\u2010to\u2010Human Cardiac Xenotransplantation","volume":"387","author":"Griffith B. P.","year":"2022","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_9_52_1","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1111\/ajt.16930","article-title":"First Clinical\u2010Grade Porcine Kidney Xenotransplant Using a Human Decedent Model","volume":"22","author":"Porrett P. M.","year":"2022","journal-title":"American Journal of Transplantation"},{"key":"e_1_2_9_53_1","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1056\/NEJMoa2120238","article-title":"Results of Two Cases of Pig\u2010to\u2010Human Kidney Xenotransplantation","volume":"386","author":"Montgomery R. A.","year":"2022","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_9_54_1","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1056\/NEJMoa2412747","article-title":"Xenotransplantation of a Porcine Kidney for End\u2010Stage Kidney Disease","volume":"392","author":"Kawai T.","year":"2025","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_9_55_1","doi-asserted-by":"crossref","first-page":"3388","DOI":"10.1038\/s41591-025-03861-x","article-title":"Pig\u2010to\u2010Human Lung Xenotransplantation Into a Brain\u2010Dead Recipient","volume":"31","author":"He J.","year":"2025","journal-title":"Nature Medicine"},{"key":"e_1_2_9_56_1","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/s41586-025-08799-1","article-title":"Gene\u2010Modified Pig\u2010to\u2010Human Liver Xenotransplantation","volume":"641","author":"Tao K.\u2010S.","year":"2025","journal-title":"Nature"},{"key":"e_1_2_9_57_1","unstructured":"eGenesis eGenesis Announces IND Clearance for EGEN\u20102784 in Kidney Transplant and Landmark Patient Updates in Ongoing Expanded Access Study.eGenesis2025."},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010025\u201009846\u20107"},{"key":"e_1_2_9_59_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/s41591-025-04053-3","article-title":"Immune Profiling in a Living Human Recipient of a Gene\u2010Edited Pig Kidney","volume":"32","author":"Ribas G. T.","year":"2026","journal-title":"Nature Medicine"},{"key":"e_1_2_9_60_1","doi-asserted-by":"crossref","DOI":"10.1111\/xen.70076","article-title":"Single\u2010Cell RNA Analysis Reveals Reduced Frequency and Activity of CXCR5+ Atypical Memory B Cells in Islet Xenotransplantation Treated With Clinically Applicable Immunosuppressants Including Belimumab","volume":"32","author":"Lee Y.","year":"2025","journal-title":"Xenotransplantation"}],"container-title":["Xenotransplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70167","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/xen.70167","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/xen.70167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:36:05Z","timestamp":1792053365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/xen.70167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":59,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/xen.70167"],"URL":"https:\/\/doi.org\/10.1111\/xen.70167","archive":["Portico"],"relation":{},"ISSN":["0908-665X","1399-3089"],"issn-type":[{"value":"0908-665X","type":"print"},{"value":"1399-3089","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70167"}}