{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:03:24Z","timestamp":1777349004762,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T00:00:00Z","timestamp":1371600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cattle faecal samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a0480) were collected from a cluster of 12 farms, and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> screened for the presence of the intimin gene (<jats:italic>eae<\/jats:italic>). Positive samples were cultured, and colonies were examined for the presence of <jats:italic>eae<\/jats:italic> and verocytotoxin (<jats:italic>vtx<\/jats:italic>) genes. Colonies which were positive for the intimin gene and negative for the verocytotoxin genes were further screened using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for a range of virulence factors including <jats:italic>bfpA<\/jats:italic>,<jats:italic> esp<\/jats:italic>A, <jats:italic>esp<\/jats:italic>B<jats:italic>, tir ehxA, toxB<\/jats:italic>,<jats:italic> etpD<\/jats:italic>,<jats:italic> katP<\/jats:italic>,<jats:italic> saa<\/jats:italic>,<jats:italic> iha<\/jats:italic>,<jats:italic> lpf<\/jats:italic>A<jats:sub>O157\/OI\u2010141<\/jats:sub> and <jats:italic>lpf<\/jats:italic>A<jats:sub>O157\/OI\u2010154.<\/jats:sub> Of the 480 faecal samples, 5.8% (28\/480) were PCR positive, and one isolate was obtained from each. All 28 isolates obtained were <jats:italic>bfpA<\/jats:italic> negative and therefore atypical <jats:styled-content style=\"fixed-case\">EPEC<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">aEPEC<\/jats:styled-content>). The serotypes detected included O2:H27, O8:H36, O15:H2, O49:H+, O84:H28, O105:H7 and O132:H34 but half of the isolates could not be serogrouped using currently available antisera. Twenty\u2010two (79%) of the isolates carried the <jats:italic>tir<\/jats:italic> gene but only 25% were <jats:italic>espB<\/jats:italic> positive, and all other virulence genes tested for were scarce or absent. Several isolates showed intermediate resistance to ciprofloxacin, kanamycin, nalidixic acid, minocycline and tetracycline; full resistance to nalidixic acid or tetracycline with one isolate (O\u2212:H8) displaying resistance to aminoglycosides (kanamycin and streptomycin), quinolones (nalidixic acid) and sulphonamides. This study provides further evidence that cattle are a potential source of <jats:styled-content style=\"fixed-case\">aEPEC<\/jats:styled-content> and add to the very limited data currently available on virulence genes and antibiotic resistance in this pathogenic <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>.\u00a0coli<\/jats:italic> group in animals.<\/jats:p>","DOI":"10.1111\/zph.12058","type":"journal-article","created":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T11:56:45Z","timestamp":1371643005000},"page":"149-156","source":"Crossref","is-referenced-by-count":17,"title":["Occurrence, Virulence Genes and Antibiotic Resistance of Enteropathogenic <i><scp>E<\/scp>scherichia coli<\/i> (<scp>EPEC<\/scp>) from Twelve Bovine Farms in the North\u2010East of <scp>I<\/scp>reland"],"prefix":"10.1111","volume":"61","author":[{"given":"D. J.","family":"Bolton","sequence":"first","affiliation":[{"name":"Department of Food Safety Teagasc Food Research Centre Ashtown Dublin 15 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ennis","sequence":"additional","affiliation":[{"name":"Department of Food Safety Teagasc Food Research Centre Ashtown Dublin 15 Ireland"},{"name":"School of Health Sciences University of Ulster Newtownabbey Co Antrim Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"McDowell","sequence":"additional","affiliation":[{"name":"School of Health Sciences University of Ulster Newtownabbey Co Antrim Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.03.025"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.10.046"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5372.2114"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46518-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0699"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-11-3305"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.10.010"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26740-0"},{"key":"e_1_2_6_10_1","volume-title":"Performance Standards for Antimicrobial Disc and Dilution Susceptibility Tests for Bacteria Isolated from Animals: Approved Standard M31\u2010A3","author":"Clinical and Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_6_11_1","article-title":"Antimicrobial resistance and virulence factors in E.\u00a0coli from Swedish dairy calves","volume":"54","author":"De Verdier K.","year":"2012","journal-title":"Acta Vet. Scan."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb02210.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/379666"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01166-08"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1683004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1128\/jcm.33.5.1375-1377.1995","article-title":"Identification of enteropathogenic E.\u00a0coli by PCR\u2010based detection of the bundle\u2010forming pilus gene","volume":"33","author":"Gunzburg S. T.","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_17_1","first-page":"233","volume-title":"Recent Advances in Verocytotoxin\u2010Producing E.\u00a0coli Infections","author":"Gyles C. L.","year":"1995"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/517342"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01664.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00013-9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.08.034"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3405-3412.2005"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01495.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.484-489.2001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(00)00223-0"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5107-5114.2001"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300114"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.2807\/ese.17.47.20321-en","article-title":"Two outbreaks of diarrhoea in nurseries in Norway after farm visits, April to May 2009","volume":"17","author":"Moller\u2010Stray J.","year":"2012","journal-title":"Euro. Surveill."},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12064"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1204.060125"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.142"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2002.01649.x"},{"key":"e_1_2_6_33_1","first-page":"667","article-title":"Serology, chemistry, and genetics of O and K antigens of E.\u00a0coli","volume":"41","author":"Orskov I.","year":"1977","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1128\/JCM.36.2.598-602.1998","article-title":"Detection and Characterization of Shiga Toxigenic E.\u00a0coli by Using Multiplex PCR Assays for stx1, stx2, eaeA, Enterohemorrhagic E.\u00a0coli hlyA, rfbO111, and rfbO157","volume":"36","author":"Paton A. W.","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6999-7009.2001"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb10299.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6863-6873.2001"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00105.x"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.11.010"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01379-7"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6853-6859.2002"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00167.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.4937-4946.2004"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0805.010385"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2940-2943.2005"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92337-H"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00709-07"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.01.023"},{"key":"e_1_2_6_49_1","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1128\/jcm.18.3.512-520.1983","article-title":"Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare E.\u00a0coli serotype","volume":"18","author":"Wells J. G.","year":"1983","journal-title":"J. Clin. Microbiol."}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fzph.12058","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.12058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T02:48:56Z","timestamp":1696042136000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.12058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,19]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/zph.12058"],"URL":"https:\/\/doi.org\/10.1111\/zph.12058","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"value":"1863-1959","type":"print"},{"value":"1863-2378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,19]]}}}