{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T05:11:28Z","timestamp":1696223488968},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T00:00:00Z","timestamp":1376697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Summary<\/jats:title>C<\/jats:styled-content>ryptosporidium<\/jats:italic> are ubiquitous and significant enteropathogens of all classes of vertebrates and a major cause of human morbidity and mortality worldwide. Of the 24 recognized species, the zoonotic C<\/jats:styled-content>ryptosporidium parvum<\/jats:italic> and the host\u2010specific C<\/jats:styled-content>ryptosporidium hominis<\/jats:italic> cause the majority of cases of human cryptosporidiosis. Here, we report on structural and transcriptional variability between C<\/jats:styled-content><\/jats:italic>.\u00a0parvum<\/jats:italic> and C<\/jats:styled-content><\/jats:italic>.\u00a0hominis<\/jats:italic> at the MIC<\/jats:styled-content>1 locus, which encodes a microneme localized thrombospondin\u2010like domain containing protein previously demonstrated to be critical for host cell infection by C<\/jats:styled-content><\/jats:italic>.\u00a0parvum<\/jats:italic>. We demonstrate, using reverse transcription quantitative PCR<\/jats:styled-content> with the aid of genomic data from the E<\/jats:styled-content>uP<\/jats:styled-content>athDB<\/jats:styled-content> site, that the transcribed product in C<\/jats:styled-content><\/jats:italic>.\u00a0hominis<\/jats:italic> is both truncated and significantly down\u2010regulated in the sporozoite. We hypothesize that C<\/jats:styled-content>pMIC<\/jats:styled-content>1 may be a genetic factor involved in facilitating the wider host range of C<\/jats:styled-content>.\u00a0parvum<\/jats:italic> in comparison with the specific host range of C<\/jats:styled-content>.\u00a0hominis<\/jats:italic>. Furthermore, we show that the presence of a microsatellite (ML<\/jats:styled-content>\u20102) within the C<\/jats:styled-content><\/jats:italic>.\u00a0parvum <\/jats:italic>MIC<\/jats:styled-content>\u20101 locus enables the development of a PCR<\/jats:styled-content> marker that can rapidly distinguish the zoonotic C<\/jats:styled-content>.\u00a0parvum<\/jats:italic> from C<\/jats:styled-content><\/jats:italic>.\u00a0hominis<\/jats:italic> and other significant human infectious C<\/jats:styled-content>ryptosporidium<\/jats:italic> species due to reproducible PCR<\/jats:styled-content> slippage across the ML<\/jats:styled-content>\u20102 microsatellite. Additionally, we demonstrate that this locus is tightly linked to the GP<\/jats:styled-content>60 locus, a locus commonly used in the genetic characterization of C<\/jats:styled-content>.\u00a0parvum<\/jats:italic> and C<\/jats:styled-content><\/jats:italic>.\u00a0hominis<\/jats:italic> isolates. This marker should provide a robust and additional tool to aid in the rapid identification of C<\/jats:styled-content>.\u00a0parvum<\/jats:italic> from other C<\/jats:styled-content>ryptosporidium<\/jats:italic> species.<\/jats:p>","DOI":"10.1111\/zph.12074","type":"journal-article","created":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T05:38:47Z","timestamp":1376717927000},"page":"324-337","source":"Crossref","is-referenced-by-count":3,"title":["PCR<\/scp> Slippage Across the ML<\/scp>\u20102 Microsatellite of the C<\/scp>ryptosporidium <\/i>MIC<\/scp>1 Locus Enables Development of a PCR<\/scp> Assay Capable of Distinguishing the Zoonotic C<\/scp>ryptosporidium parvum<\/i> From Other Human Infectious C<\/scp>ryptosporidium<\/i> Species"],"prefix":"10.1111","volume":"61","author":[{"given":"M. A.","family":"Webber","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology School of Medicine Flinders University Bedford Park SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Sari","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Medical Sciences University of South Australia Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Hoefel","sequence":"additional","affiliation":[{"name":"Australian Water Quality Centre South Australian Water Corporation Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. T.","family":"Monis","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology School of Medicine Flinders University Bedford Park SA Australia"},{"name":"School of Pharmacy and Medical Sciences University of South Australia Adelaide SA Australia"},{"name":"Australian Water Quality Centre South Australian Water Corporation Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. J.","family":"King","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology School of Medicine Flinders University Bedford Park SA Australia"},{"name":"Australian Water Quality Centre South Australian Water Corporation Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00233-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/519842"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04764.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/ese.14.02.19086-en","article-title":"Long\u2010term Cryptosporidium typing reveals the aetiology and species\u2010specific epidemiology of human cryptosporidiosis in England and Wales, 2000 to 2003","volume":"14","author":"Chalmers R. M.","year":"2009","journal-title":"Euro. Surveill."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1703.100410"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2009.11.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6987-6995.2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01254.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.03.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.04.028"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113369"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"pII 20318","DOI":"10.2807\/ese.17.46.20318-en","article-title":"Two geographically separated food\u2010borne outbreaks in Sweden linked by an unusual Cryptosporidium parvum subtype, October 2010","volume":"17","author":"Gherasim A.","year":"2012","journal-title":"Euro. Surveill."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006632"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj078"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01636-08"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.Supplement_1.S57"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2505-2511.2003"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006001491"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3848-3857.2005"},{"key":"e_1_2_7_22_1","volume-title":"Identification of Genes Expressed During Excystation, Pre\u2010Infection and Early Infection as Markers of Infectivity in Cryptosporidium","author":"King B. J.","year":"2009"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011000217"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0772-8"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1674-8301(11)60001-8"},{"key":"e_1_2_7_26_1","volume-title":"Cryptosporidium in \u00d6stersund during the Winter 2010\/2011: Consequences and Costs from an Outbreak of a Waterborne Disease","author":"Lindberg A.","year":"2011"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199407213310304"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031030"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2002.tb00224.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00305-08"},{"key":"e_1_2_7_31_1","first-page":"E070503 070503","article-title":"A large outbreak of cryptosporidiosis in western Ireland linked to public water supply: a preliminary report","volume":"12","author":"Pelly H.","year":"2007","journal-title":"Euro. Surveill."},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.09.004"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00751-11"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002567"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2009.01308.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2012.10.003"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012001151"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg178"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.14.2760"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00225-X"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2008.02.009"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318235ab64"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4943"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-312"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.03.027"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00377.x"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5492-5498.2000"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02977"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fzph.12074","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.12074","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T17:12:01Z","timestamp":1696180321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.12074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,17]]},"references-count":47,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/zph.12074"],"URL":"https:\/\/doi.org\/10.1111\/zph.12074","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"value":"1863-1959","type":"print"},{"value":"1863-2378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,17]]}}}