{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:30:07Z","timestamp":1790670607016,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T00:00:00Z","timestamp":1384473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001504","name":"Ministry of Health, New Zealand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001504","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study describes the analysis of 432 isolates of <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>ersinia enterocolitica<\/jats:italic> by pulsed\u2010field gel electrophoresis (<jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> had a high level of discrimination with biotype 1A isolates (Simpson's Diversity Index 0.997), but with the clinically important biotypes 2, 3 and 4, the discriminatory ability of <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> was so low as to severely limit its usefulness (<jats:styled-content style=\"fixed-case\">DI<\/jats:styled-content> &lt;0.6). For biotypes 2, 3 and 4, 79% or more of isolates of each biotype were of just three different <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> profiles. Because of this, four known outbreaks of yersiniosis would not have been identified by <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> analysis. However, a previously unrecognized potential outbreak of yersiniosis caused by biotype 4 isolates was identified on the basis of a rare <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> genotype with spatial and temporal clustering. We conclude that <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> has a very limited application to the genotyping of <jats:italic>Y.\u00a0enterocolitica<\/jats:italic> biotypes 2, 3 and 4, and inferences based on finding indistinguishable <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content> profiles among cases or between cases and sources need to be substantiated using alternative typing tools, or strong epidemiological evidence.<\/jats:p>","DOI":"10.1111\/zph.12085","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T09:48:40Z","timestamp":1384508920000},"page":"405-410","source":"Crossref","is-referenced-by-count":27,"title":["The Limitations of Pulsed\u2010Field Gel Electrophoresis for Analysis of <i><scp>Y<\/scp>ersinia enterocolitica<\/i> Isolates"],"prefix":"10.1111","volume":"61","author":[{"given":"B. J.","family":"Gilpin","sequence":"first","affiliation":[{"name":"Institute of Environmental Science and Research Christchurch Science Centre Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Robson","sequence":"additional","affiliation":[{"name":"Institute of Environmental Science and Research Christchurch Science Centre Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Environmental Science and Research Christchurch Science Centre Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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