{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T16:34:38Z","timestamp":1781627678700,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,5,27]],"date-time":"2014-05-27T00:00:00Z","timestamp":1401148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Dobrava\u2013Belgrade virus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content>) is a hantavirus species that causes the most severe form of haemorrhagic fever with renal syndrome (<jats:styled-content style=\"fixed-case\">HFRS<\/jats:styled-content>) in Europe. <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> has been detected in three <jats:italic>Apodemus<\/jats:italic> rodents: <jats:italic>A.\u00a0flavicollis<\/jats:italic>,<jats:italic> A.\u00a0agrarius<\/jats:italic> and <jats:italic>A.\u00a0ponticus<\/jats:italic>. These emerging viruses appear throughout the Balkan Peninsula including Serbia as its central part. In this study, we examined the seroprevalence, molecular epidemiology and phylogenetics of <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> from <jats:italic>A.\u00a0flavicollis<\/jats:italic> captured at six Serbian localities. Furthermore, we applied microsatellite typing of host animal genome to analyse the role of host kinship in <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> animal transmission. The overall IgG seropositivity rate over 3\u00a0years (2008\u20132010) was 11.9% (22\/185). All seropositive samples were subjected to <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequencing for S and L genome segments (pos. 291\u20131079\u00a0nt and 2999\u20133316\u00a0nt, respectively). <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> was genetically detected in three samples from mountain Tara in western Serbia, a newly detected <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> focus in the Balkans. No sequence data from human cases from Serbia are available for the studied period. However, collected <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> isolates in this work phylogenetically clustered together with isolates from Serbian human cases dating from 2002, with 1.9% nucleotide divergence. We determined the level of kinship between seropositive and seronegative animal groups and found no significant difference, suggesting that horizontal virus transmission in the studied population was the same within and among the hatches. Our findings are the first genetic detection of <jats:styled-content style=\"fixed-case\">DOBV<\/jats:styled-content> in rodents in Serbia. We confirm wide and continuous hantavirus presence in the examined parts of the Balkans, underlying the necessity of continual monitoring of hantavirus circulation in <jats:italic>A.\u00a0flavicollis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/zph.12136","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T02:39:11Z","timestamp":1401244751000},"page":"141-150","source":"Crossref","is-referenced-by-count":7,"title":["Genetic Analysis of <i>Dobrava\u2013Belgrade Virus<\/i> from Western Serbia \u2013 A Newly Detected Focus in the Balkan Peninsula"],"prefix":"10.1111","volume":"62","author":[{"given":"G.","family":"Stamenkovi\u0107","sequence":"first","affiliation":[{"name":"Department of Genetic Research Institute for biological research \u201cSini\u0161a Stankovi\u0107\u201d University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Nikoli\u0107","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Immunology Faculty of Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Blagojevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research Institute for biological research \u201cSini\u0161a Stankovi\u0107\u201d University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Bugarski\u2010Stanojevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research Institute for biological research \u201cSini\u0161a Stankovi\u0107\u201d University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Adna\u0111evi\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research Institute for biological research \u201cSini\u0161a Stankovi\u0107\u201d University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Stanojevi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Immunology Faculty of Medicine University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Vujo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Department of Genetic Research Institute for biological research \u201cSini\u0161a Stankovi\u0107\u201d University of Belgrade Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890380211"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-7-1747"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14772000.2011.560970"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2012.01532.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-007-0005-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.1.204"},{"key":"e_1_2_8_8_1","first-page":"17","volume-title":"Hemorrhagic Fever with Renal Syndrome","author":"Gligi\u0107 A.","year":"2008"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365548809032449"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.41.109"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.1.113"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.1997.00222.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03193121"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003159"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294x.2000.01043-3.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00062-09"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2006.01256.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-005-0555-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1205.051487"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1514-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-004-0414-8"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199709)53:1<51::AID-JMV9>3.0.CO;2-P"},{"key":"e_1_2_8_26_1","volume-title":"Mammals of Britain and Europe","author":"Macdonald D. 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