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In the 2008\/2009 season, this abattoir experienced three human leptospirosis cases from 20 staff, of which two were hospitalized. Urine, kidney and blood samples were collected from carcasses of 399 sheep (six suppliers, 17 slaughter lines) and 146 cattle (three suppliers, 22 slaughter lines). The urine and kidney samples were tested by quantitative real\u2010time PCR (q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>), while serum samples (from coagulated blood samples) were tested by microscopic agglutination test (<jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>). In total, 27% (73\/274; 95% CI: 18\u201337) of urine samples tested positive by q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Species\u2010specific shedding rates (prevalence of positive urine q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) were 31% (95% CI: 17\u201348) for sheep and 21% (95% CI: 14\u201330) for cattle. For 545 kidney samples tested, 145 were q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>positive (27%; 95% CI: 17\u201339). The average prevalence of kidney q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>positivity was 29% (95% CI: 17\u201345) for sheep and 21% (95% CI: 15\u201328) for cattle. Three hundred and thirty of 542 sampled sheep and cattle had antibodies against<jats:italic>Leptospira borgpetersenii<\/jats:italic>serovar Hardjobovis (Hardjobovis) and\/or<jats:italic>Leptospira interrogans<\/jats:italic>serovar Pomona (Pomona), based on reciprocal<jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>titre \u22651\u00a0:\u00a048 (overall seroprevalence of 61%; 95% CI: 48\u201373). Seroprevalence was 57% (95% CI: 40\u201372) for sheep and 73% (95% CI: 59\u201383) for cattle. Among the seropositive animals, 41% (70\/170; 95% CI: 30\u201354) were shedding (tested positive by urine q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and 42% (137\/330; 95% CI: 30\u201354) had renal carriage (tested positive by kidney q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Some risk management options for abattoirs or farms to prevent human leptospirosis infections include vaccination of maintenance hosts, the use of personal protective equipment, and the application of urine q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>to detect shedding status of stock as surveillance and as an alert.<\/jats:p>","DOI":"10.1111\/zph.12146","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T13:48:18Z","timestamp":1404827298000},"page":"258-268","source":"Crossref","is-referenced-by-count":43,"title":["Shedding and Seroprevalence of Pathogenic<i>Leptospira<\/i>spp. in Sheep and Cattle at a New Zealand Abattoir"],"prefix":"10.1111","volume":"62","author":[{"given":"F.","family":"Fang","sequence":"first","affiliation":[{"name":"Molecular Epidemiology and Veterinary Public Health Laboratory (<sup>m<\/sup>EpiLab) and Infectious Disease Research Centre (IDReC) Hopkirk Research Institute Massey University Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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