{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:17:02Z","timestamp":1781245022871,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Brazilian Council for Scientific and Technological Development (CNPq)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to investigate the phenotypic and genotypic diversity and anti\u2010microbial resistance among staphylococci of dairy herds that originated from <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>araiba <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>tate, north\u2010eastern <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>razil, a region where such studies are rare. Milk samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a0552) were collected from 15 dairy farms. Isolates were evaluated for anti\u2010microbial susceptibility by <jats:styled-content style=\"fixed-case\">Kirby<\/jats:styled-content>\u2013Bauer disc diffusion method. Confirmation of methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) was performed using multiplex PCR targeting <jats:italic>mecA<\/jats:italic> and <jats:italic>nuc<\/jats:italic> genes in addition to phenotypic assay based on PBP\u20102a latex agglutination. Clonal relatedness of isolates was determined by pulsed\u2010field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) genotyping. Staphylococci were detected in 269 (49%) of the samples. Among these, 65 (24%) were <jats:italic>S.\u00a0aureus<\/jats:italic>. The remaining 204 isolates were either coagulase\u2010negative staphylococci (<jats:italic>n<\/jats:italic>\u00a0=\u00a0188; 70%) or coagulase positive other than <jats:italic>S.\u00a0aureus<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a016; 6%). Staphylococci were cultured in seven (35%) of the 20 hand swab samples, from which five isolates were <jats:italic>S.\u00a0aureus<\/jats:italic>. The isolates were most commonly resistant against penicillin (43%), ampicillin (38%) and oxacillin (27%). The gene <jats:italic>mecA<\/jats:italic> was detected in 21 <jats:italic>S.\u00a0aureus<\/jats:italic> from milk and in one isolate from a milker's hand. None of the isolates were resistant to vancomycin. PFGE findings showed high clonal diversity among the isolates. Based on <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>, we identified a total of 11 different sequence types (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s 1, 5, 6, 83, 97, 126, 1583, 1622, 1623, 1624 and 1625) with four novel <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>s (<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>1622\u2010<jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>1625). The findings show that <jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content> is prevalent in milk from semi\u2010extensive dairy cows in north\u2010eastern Brazil, and further investigation on its extent in various types of milk production systems and the farm\u2010to\u2010table continuum is warranted.<\/jats:p>","DOI":"10.1111\/zph.12209","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T14:06:23Z","timestamp":1436882783000},"page":"97-105","source":"Crossref","is-referenced-by-count":25,"title":["Methicillin\u2010Resistant <i>Staphylococcus aureus<\/i> from Brazilian Dairy Farms and Identification of Novel Sequence Types"],"prefix":"10.1111","volume":"63","author":[{"given":"C. J. B.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Department of Animal Science Centre for Agricultural Sciences Federal University of Paraiba Areia PB Brazil"},{"name":"Veterinary Public Health and Biotechnology Global Consortium (VPH\u2010Biotec) The Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Tiao","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine College of Veterinary Medicine The Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. G. C.","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Department of Animal Science Centre for Agricultural Sciences Federal University of Paraiba Areia PB Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F. P.","family":"de Moura","sequence":"additional","affiliation":[{"name":"Department of Animal Science Centre for Agricultural Sciences Federal University of Paraiba Areia PB Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Santos Filho","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Center for Health Sciences Federal University of Paraiba Jo\u00e3o Pessoa PB Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. A.","family":"Gebreyes","sequence":"additional","affiliation":[{"name":"Veterinary Public Health and Biotechnology Global Consortium (VPH\u2010Biotec) The Ohio State University Columbus OH USA"},{"name":"Department of Preventive Veterinary Medicine College of Veterinary Medicine The Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00019-07"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.7.1185"},{"key":"e_1_2_7_4_1","volume-title":"CLSI. 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