{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T15:26:10Z","timestamp":1759159570106},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T00:00:00Z","timestamp":1437350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000094","name":"Ontario Ministry of Agriculture, Food and Rural Affairs","doi-asserted-by":"publisher","award":["Grant # 027118"],"award-info":[{"award-number":["Grant # 027118"]}],"id":[{"id":"10.13039\/501100000094","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We collected 599 Canadian retail pork chops and 283 pork livers routinely (usually weekly) from April 2011 to March 2012 using the Canadian Integrated Program for Antimicrobial Resistance Surveillance (<jats:styled-content style=\"fixed-case\">CIPARS<\/jats:styled-content>) retail sampling platform. Samples were assayed using validated real\u2010time (q) reverse transcriptase polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and nested classical <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for the detection of hepatitis E virus (<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>), porcine enteric calicivirus (<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content>) and rotavirus (<jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content>). The presence of <jats:italic>Escherichia coli<\/jats:italic>,<jats:italic> Salmonell<\/jats:italic>a spp. and <jats:italic>Campylobacter<\/jats:italic> spp. was measured on a subset of our samples. Exact logistic regression models were fitted for predictors for <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> detection, for each assay. For both assays, sample type (pork chop versus liver) was a significant predictor for <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> detection. For nested classical <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> but not <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, region of sample collection was a significant predictor (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.008) of <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> detection. Odds of <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> detection were greatest in spring relative to other seasons. <jats:italic>E.\u00a0coli<\/jats:italic> was a significant predictor for <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> detection using the <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.03). Overall, the prevalence of <jats:italic>E.\u00a0coli<\/jats:italic>,<jats:italic> Salmonella<\/jats:italic> spp. and <jats:italic>Campylobacter<\/jats:italic> spp. was significantly greater than <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PEC<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content> on our retail pork samples. Our sparse data set for the detection of <jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RV<\/jats:styled-content> precluded modelling of risk factors for the detection of these viruses.<\/jats:p>","DOI":"10.1111\/zph.12216","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T13:49:30Z","timestamp":1437400170000},"page":"152-159","source":"Crossref","is-referenced-by-count":7,"title":["Factors Affecting Detection of Hepatitis E Virus on Canadian Retail Pork Chops and Pork Livers Assayed Using Real\u2010Time <scp>RT<\/scp>\u2010<scp>PCR<\/scp>"],"prefix":"10.1111","volume":"63","author":[{"given":"B. 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