{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T03:21:51Z","timestamp":1768620111234,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T00:00:00Z","timestamp":1446681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008430","name":"Fundaci\u00f3n para el Fomento en Asturias de la Investigaci\u00f3n Cient\u00edfica Aplicada y la Tecnolog\u00eda","doi-asserted-by":"crossref","award":["FICYT-BP11-050"],"award-info":[{"award-number":["FICYT-BP11-050"]}],"id":[{"id":"10.13039\/501100008430","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fondo de Investigaci\u00f3n Sanitaria"},{"name":"Instituto de Salud Carlos III of Spain"},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Salmonella enterica<\/jats:italic> subsp. <jats:italic>enterica<\/jats:italic> serovar Eppendorf, with antigenic formula 1,4,12,[27]:d:1,5, is an infrequent serovar. However, 14% (20 of 142) of the isolates recovered during June\u2013July 2012 in chicken farms in Tunisia belonged to <jats:italic>S<\/jats:italic>. Eppendorf. These isolates were analysed for resistance and virulence profiles. None of them were susceptible to all antimicrobials tested, while 70%, 60%, 50%, 50%, 20% and 5% were resistant to sulphonamides (<jats:italic>sul1<\/jats:italic>,<jats:italic> sul2<\/jats:italic> and <jats:italic>sul3<\/jats:italic>), streptomycin (<jats:italic>aadA1<\/jats:italic>\u2010like), trimethoprim (<jats:italic>dfrA1<\/jats:italic>\u2010like), nalidixic acid (GyrA Asp<jats:sub>87<\/jats:sub>\u2192Asn and not identified), gentamicin (not identified) and ampicillin (<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>\u20101\u2010like). About 30% of the isolates showed decreased susceptibility to ciprofloxacin and carried the <jats:italic>qnrB<\/jats:italic> gene; 65% of the isolates were multidrug resistant and contained class 1 integrons with <jats:italic>sul1<\/jats:italic> or <jats:italic>sul3<\/jats:italic> in the 3\u2032 conserved segment. The <jats:italic>orgA<\/jats:italic>,<jats:italic> ssaQ<\/jats:italic>,<jats:italic> mgtC<\/jats:italic>,<jats:italic> siiD<\/jats:italic> and <jats:italic>sopB<\/jats:italic> virulence genes located on SPI1 to SPI5 and the fimbrial <jats:italic>bcfC<\/jats:italic> gene were present in all isolates; the <jats:italic>sopE1<\/jats:italic> and <jats:italic>sodC1<\/jats:italic> carried by prophages were variably detected; however, the prophage <jats:italic>gipA<\/jats:italic> gene and the <jats:italic>spvC<\/jats:italic> gene of serovar\u2010specific virulence plasmids were absent. Altogether, ten resistance and three virulence profiles were identified. Typing of the isolates with <jats:italic>Xba<\/jats:italic>I\u2010 and <jats:italic>Bln<\/jats:italic>I\u2010PFGE supports a close relationship, although they appear to be evolving under selective pressure probably caused by antimicrobial use in chicken husbandry. As far as we know, this is the first study investigating the molecular bases of antimicrobial drug resistance, the virulence gene content and the PFGE profiles of <jats:italic>S<\/jats:italic>. Eppendorf. The epidemiological surveillance of this serovar would be necessary to evaluate its possible impact on human health, particularly in Tunisia and other African countries where it was already reported.<\/jats:p>","DOI":"10.1111\/zph.12234","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T12:51:03Z","timestamp":1446727863000},"page":"320-327","source":"Crossref","is-referenced-by-count":5,"title":["Detection and Molecular Characterization of <i>Salmonella enterica<\/i> Serovar Eppendorf Circulating in Chicken Farms in Tunisia"],"prefix":"10.1111","volume":"63","author":[{"given":"R.","family":"Ben Salem","sequence":"first","affiliation":[{"name":"Veterinary Research Institute of Tunisia Tunis Tunisia"},{"name":"Faculty of Sciences of Bizert University of Carthage Bizert Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.S.","family":"Abbassi","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute of Tunisia Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional \u00c1rea de Microbiolog\u00eda Universidad de Oviedo Oviedo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Garc\u00eda\u2010Fierro","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional \u00c1rea de Microbiolog\u00eda Universidad de Oviedo Oviedo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Njoud","sequence":"additional","affiliation":[{"name":"Regional Center of Veterinary Research Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Messadi","sequence":"additional","affiliation":[{"name":"National School of Veterinary Medicine Department of Microbiology and Immunology University of La Manouba Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional \u00c1rea de Microbiolog\u00eda Universidad de Oviedo Oviedo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2005.pto960401.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2012.1466"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01275-06"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.5065"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.20"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00425-11"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.12.017"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-3-9"},{"key":"e_1_2_7_10_1","unstructured":"Clinical and Laboratory Standards Institute (C. 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