{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T17:14:58Z","timestamp":1751476498113},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T00:00:00Z","timestamp":1470182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The dissemination of <jats:italic>Enterobacteriaceae<\/jats:italic> expressing resistance to extended\u2010spectrum cephalosporins, which are therapeutically used in both human and veterinary medicine, is of critical concern. The normal commensal flora of food animals may serve as an important reservoir for the zoonotic food\u2010borne transmission of <jats:italic>Enterobacteriaceae<\/jats:italic> harbouring \u03b2\u2010lactam resistance. We hypothesized that the predominant AmpC and <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content> genes reported in <jats:styled-content style=\"fixed-case\">US<\/jats:styled-content> livestock and fresh retail meat products, <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CMY<\/jats:sub><\/jats:styled-content><jats:sub>\u20102<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CTX<\/jats:sub><\/jats:styled-content><jats:sub>\u2010M<\/jats:sub>, would also be predominant in human enteric flora. We recovered enteric flora from a convenience sample of patients included in a large tertiary medical centre's <jats:italic>Clostridium difficile<\/jats:italic> surveillance programme to screen for and estimate the frequency of carriage of AmpC and <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content> resistance genes. In\u2010 and outpatient diarrhoeic submissions (<jats:italic>n<\/jats:italic>\u00a0=\u00a0692) received for <jats:italic>C.\u00a0difficile<\/jats:italic> testing at the medical centre's clinical diagnostic laboratory from July to December, 2013, were included. Aliquoted to a transport swab, each submission was inoculated to MacConkey broth with cefotaxime, incubated at 37\u00b0C and then inoculated to MacConkey agars supplemented with cefoxitin and cefepime to select for the AmpC and <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content> phenotypes, with <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CMY<\/jats:sub><\/jats:styled-content> and <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CTX<\/jats:sub><\/jats:styled-content><jats:sub>\u2010M<\/jats:sub> genotypes confirmed by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and sequencing. From the 692 diarrhoeic submissions, our selective culture yielded 184 isolates (26.6%) with reduced susceptibility to cefotaxime. Of these, 46 (6.7%) samples harboured commensal isolates carrying the AmpC <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CMY<\/jats:sub><\/jats:styled-content>. Another 21 (3.0%) samples produced isolates harbouring the <jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content> <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sub>CTX<\/jats:sub><\/jats:styled-content><jats:sub>\u2010M<\/jats:sub>: 19 carrying <jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content>\u2010M\u201015 and 2 with <jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content>\u2010M\u201027. Our results indicate that \u03b2\u2010lactam resistance genes likely acquired through zoonotic food\u2010borne transmission are present in the enteric flora of this hospital\u2010associated population at lower levels than reported in livestock and fresh food products.<\/jats:p>","DOI":"10.1111\/zph.12293","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T11:26:18Z","timestamp":1470223578000},"page":"161-164","source":"Crossref","is-referenced-by-count":5,"title":["Extended\u2010Spectrum \u03b2\u2010lactam Resistance in the Enteric Flora of Patients at a Tertiary Care Medical Centre"],"prefix":"10.1111","volume":"64","author":[{"given":"T. F.","family":"Landers","sequence":"first","affiliation":[{"name":"The Ohio State University College of Nursing Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. F.","family":"Mollenkopf","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine The Ohio State University College of Veterinary Medicine Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"Faubel","sequence":"additional","affiliation":[{"name":"Department of Pathology The Ohio State University Wexner Medical Center Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Dent","sequence":"additional","affiliation":[{"name":"The Ohio State University College of Nursing Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pancholi","sequence":"additional","affiliation":[{"name":"Department of Pathology The Ohio State University Wexner Medical Center Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. B.","family":"Daniels","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences The Ohio State University College of Veterinary Medicine Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. E.","family":"Wittum","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine The Ohio State University College of Veterinary Medicine Columbus OH USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/107662903322762815"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00110"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02252-12"},{"key":"e_1_2_7_5_1","first-page":"1162","volume-title":"Extended\u2010Spectrum Cephalosporin Resistance Genes in Escherichia coli from Beef Cattle","author":"Cormier A. 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