{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T20:58:48Z","timestamp":1761253128503},"reference-count":97,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T00:00:00Z","timestamp":1481673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The spirochaete (<jats:italic>Borrelia burgdorferi<\/jats:italic>) associated with Lyme disease was detected in questing ticks and rodents during a period of 18\u00a0years, 1991\u20132009, at five locations on the Outer Banks of North Carolina. The black\u2010legged tick (<jats:italic>Ixodes scapularis<\/jats:italic>) was collected at varied intervals between 1991 and 2009 and examined for <jats:italic>B.\u00a0burgdorferi<\/jats:italic>. The white\u2010footed mouse (<jats:italic>Peromyscus leucopus<\/jats:italic>), house mouse (<jats:italic>Mus musculus<\/jats:italic>) marsh rice rat (<jats:italic>Oryzomys palustris<\/jats:italic>), marsh rabbit (<jats:italic>Sylvilagus palustris<\/jats:italic>), eastern cottontail (<jats:italic>Sylvilagus floridanus<\/jats:italic>) and six\u2010lined racerunner (<jats:italic>Cnemidophorus sexlineatus<\/jats:italic>) were live\u2010trapped, and their tissues cultured to isolate spirochaetes. <jats:italic>Borrelia burgdorferi<\/jats:italic> isolates were obtained from questing adult <jats:italic>I<\/jats:italic>.\u00a0<jats:italic>scapularis<\/jats:italic> and engorged <jats:italic>I.\u00a0scapularis<\/jats:italic> removed from <jats:italic>P.\u00a0leucopus<\/jats:italic>,<jats:italic> O.\u00a0palustris<\/jats:italic> and <jats:italic>S.\u00a0floridanus<\/jats:italic>. The prevalence of <jats:italic>B.\u00a0burgdorferi<\/jats:italic> infection was variable at different times and sites ranging from 7 to 14% of examined questing <jats:italic>I.\u00a0scapularis<\/jats:italic>. Mitochondrial (16S) <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene phylogenetic analysis from 65 adult <jats:italic>I.\u00a0scapularis<\/jats:italic> identified 12 haplotypes in two major clades. Nine haplotypes were associated with northern\/Midwestern <jats:italic>I.\u00a0scapularis<\/jats:italic> populations and three with southern <jats:italic>I.\u00a0scapularis<\/jats:italic> populations. Sixteen isolates obtained from tick hosts in 2005 were confirmed to be <jats:italic>B.\u00a0burgdorferi<\/jats:italic> by amplifying and sequencing of 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> and 5S\u201023S intergenic spacer fragments. The sequences had 98\u201399% identity to <jats:italic>B.\u00a0burgdorferi<\/jats:italic> sensu stricto strains B31, <jats:styled-content style=\"fixed-case\">JD<\/jats:styled-content>1 and M11p. Taken together, these studies indicate that <jats:italic>B.\u00a0burgdorferi<\/jats:italic> sensu stricto is endemic in questing <jats:italic>I<\/jats:italic>.\u00a0<jats:italic>scapularis<\/jats:italic> and mammalian tick hosts on the Outer Banks of North Carolina.<\/jats:p>","DOI":"10.1111\/zph.12302","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T13:50:32Z","timestamp":1481809832000},"page":"337-354","source":"Crossref","is-referenced-by-count":8,"title":["Stable Transmission of <i>Borrelia burgdorferi<\/i> Sensu Stricto on the Outer Banks of North Carolina"],"prefix":"10.1111","volume":"64","author":[{"given":"J. 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R.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine North Carolina State University Raleigh NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Kakumanu","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture and Life Sciences North Carolina State University Raleigh NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ponnusamy","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture and Life Sciences North Carolina State University Raleigh NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sutton","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture and Life Sciences North Carolina State University Raleigh NC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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