{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:02:13Z","timestamp":1790427733634,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T00:00:00Z","timestamp":1501200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Campylobacter jejuni<\/jats:italic> is among the most prevalent causes of human bacterial gastroenteritis worldwide. Domesticated animals and, especially, chicken meat are considered to be the main sources of infections. However, the contribution of surface waters and wildlife in <jats:italic>C. jejuni<\/jats:italic> transmission to humans is not well understood. We have evaluated the source attribution potential of a six\u2010gene multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">mPCR<\/jats:styled-content>) method coupled with <jats:styled-content style=\"fixed-case\">STRUCTURE<\/jats:styled-content> analysis on a set of 410 <jats:italic>C. jejuni<\/jats:italic> strains isolated from environment, livestock, food and humans in central Europe. Multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> fingerprints were analysed using Subclade prediction algorithm to classify them into six distinct <jats:styled-content style=\"fixed-case\">mPCR<\/jats:styled-content> clades. A subset of <jats:italic>C. jejuni<\/jats:italic> isolates (70%) was characterized by multilocus sequence typing (<jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>) demonstrating 74% congruence between <jats:styled-content style=\"fixed-case\">mPCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content>. The correspondence analysis of <jats:styled-content style=\"fixed-case\">mPCR<\/jats:styled-content> clades and sources of isolation indicated three distinct groups in the studied <jats:italic>C. jejuni<\/jats:italic> population\u2014the first one associated with isolates from poultry, the second one with isolates from cattle, and the third one with isolates from the environment. The <jats:styled-content style=\"fixed-case\">STRUCTURE<\/jats:styled-content> analysis attributed 7.2% and 21.7% of human isolates to environmental sources based on <jats:styled-content style=\"fixed-case\">MLST<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">mPCR<\/jats:styled-content> fingerprints, respectively.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.12366","type":"journal-article","created":{"date-parts":[[2017,7,29]],"date-time":"2017-07-29T03:36:36Z","timestamp":1501299396000},"page":"51-58","source":"Crossref","is-referenced-by-count":17,"title":["Population structure and attribution of human clinical <i>Campylobacter jejuni<\/i> isolates from central Europe to livestock and environmental sources"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9465-4552","authenticated-orcid":false,"given":"J.","family":"Kovac","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"},{"name":"Department of Food Science The Pennsylvania State University  State College PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Stessl","sequence":"additional","affiliation":[{"name":"Institute of Milk Hygiene Milk Technology and Food Science Department for Farm Animals and Veterinary Public Health University of Veterinary Medicine  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"\u010cade\u017e","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Gruntar","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Parasitology Veterinary Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cimerman","sequence":"additional","affiliation":[{"name":"National Laboratory of Health Environment and Food Department of Microbiological Analysis of Food Water and Environmental Samples Maribor  Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Stingl","sequence":"additional","affiliation":[{"name":"Department of Biological Safety National Reference Laboratory for Campylobacter Federal Institute for Risk Assessment (BfR)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lu\u0161icky","sequence":"additional","affiliation":[{"name":"National Laboratory of Health Environment and Food Department of Microbiological Analysis of Food Water and Environmental Samples Maribor  Maribor Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ocepek","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Parasitology Veterinary Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Milk Hygiene Milk Technology and Food Science Department for Farm Animals and Veterinary Public Health University of Veterinary Medicine  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Smole Mo\u017eina","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503252102"},{"key":"e_1_2_7_3_1","first-page":"776","article-title":"Insect infection model for Campylobacter jejuni reveals that O\u2010methyl phosphoramidate has insecticidal activity","volume":"201","author":"Champion O. 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