{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:46:13Z","timestamp":1790419573985,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T00:00:00Z","timestamp":1499904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"U.S. Department of Agriculture National Research Initiative USDA -NIFA-AFRI","award":["2011-67005-30182"],"award-info":[{"award-number":["2011-67005-30182"]}]},{"name":"U.S. Department of Agriculture National Research Initiative USDA -NIFA-AFRI","award":["1 I01 CX000192 01"],"award-info":[{"award-number":["1 I01 CX000192 01"]}]},{"name":"Office of Research and Development, Medical Research Service, Department of Veterans Affairs","award":["2011-67005-30182"],"award-info":[{"award-number":["2011-67005-30182"]}]},{"name":"Office of Research and Development, Medical Research Service, Department of Veterans Affairs","award":["1 I01 CX000192 01"],"award-info":[{"award-number":["1 I01 CX000192 01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The zoonotic potential of <jats:italic>Escherichia coli<\/jats:italic> from chicken\u2010source food products is important to define for public health purposes. Previously, genotypic and phenotypic screening of <jats:italic>E.\u00a0coli<\/jats:italic> isolates from commercial chicken meat and shell eggs identified some <jats:italic>E.\u00a0coli<\/jats:italic> strains that by molecular criteria resembled human\u2010source extraintestinal pathogenic <jats:italic>E.\u00a0coli<\/jats:italic> (Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content>). Here, to clarify the zoonotic risk of such chicken\u2010source <jats:italic>E.\u00a0coli<\/jats:italic>, we compared selected <jats:italic>E.\u00a0coli<\/jats:italic> isolates from chicken meat and eggs, stratified by molecularly defined Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> status, to human\u2010source Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> and to laboratory <jats:italic>E.\u00a0coli<\/jats:italic> for virulence in rodent models of sepsis, meningitis and <jats:styled-content style=\"fixed-case\">UTI<\/jats:styled-content>, and evaluated whether specific bacterial characteristics predict experimental virulence. Multiple chicken\u2010source <jats:italic>E.\u00a0coli<\/jats:italic> resembled human\u2010source Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> in their ability to cause one or multiple different Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content>\u2010associated infections. Swimming ability corresponded with urovirulence, K1 capsule corresponded with ability to cause neonatal meningitis, and biofilm formation in urine corresponded with ability to cause sepsis. In contrast, molecularly defined Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> status and individual genotypic traits were uncorrelated with ability to cause sepsis, and neither complement sensitivity nor growth in human urine corresponded with virulence in any infection model. These findings establish that chicken\u2010derived food products contain <jats:italic>E.\u00a0coli<\/jats:italic> strains that, in rodent models of multiple human\u2010associated Ex<jats:styled-content style=\"fixed-case\">PEC<\/jats:styled-content> infections, are able to cause disease comparably to human\u2010source <jats:italic>E.\u00a0coli<\/jats:italic> clinical isolates, which suggests that they may pose a significant food safety threat. Further study is needed to define the level of risk they pose to human health, which if appreciable would justify efforts to monitor for and reduce or eliminate them.<\/jats:p>","DOI":"10.1111\/zph.12376","type":"journal-article","created":{"date-parts":[[2017,7,13]],"date-time":"2017-07-13T12:28:17Z","timestamp":1499948897000},"page":"103-113","source":"Crossref","is-referenced-by-count":56,"title":["<i>Escherichia coli<\/i> isolates from commercial chicken meat and eggs cause sepsis, meningitis and urinary tract infection in rodent models of human infections"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4967-7173","authenticated-orcid":false,"given":"M.","family":"Mellata","sequence":"first","affiliation":[{"name":"The Biodesign Institute Arizona State University  Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"R.","family":"Curtiss","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University  Tempe AZ USA"},{"name":"School of Life Sciences Arizona State University  Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000448"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2009.12.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/681473"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.20.6061"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2000.01211.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600938103"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1407"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2265"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2015.04.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1531-z"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030154"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.11.4470-4475.1995"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01874-10"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00281-10"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0266"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofv083"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06375-11"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2405"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115965"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1091914"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e318250aa72"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607898104"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7644-7656.2005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-009-1738-3"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1606567113"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-54-64"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis502"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.UTI-0006-2012"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2007.0026"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03524-14"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.4161\/21505594.2014.991234"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000303"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2015.05.006"},{"key":"e_1_2_8_35_1","first-page":"58157","article-title":"Shiga toxin\u2010producing Escherichia coli in certain raw beef products","volume":"76","author":"Register F.","year":"2011","journal-title":"Federal Register"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2015.05.012"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5306-5314.1999"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1086\/315418"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.180190.114"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00363-06"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2007.0087"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086565"},{"key":"e_1_2_8_43_1","first-page":"pii: 2070","article-title":"Transurethral induction of mouse urinary tract infection","volume":"42","author":"Thai K. 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