{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:35:16Z","timestamp":1770327316067,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,9,17]],"date-time":"2017-09-17T00:00:00Z","timestamp":1505606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Cooperative Research Centre for High Integrity Pork","award":["2A-106"],"award-info":[{"award-number":["2A-106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To investigate public health implications of antibiotics to control post\u2010weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre\u2010 and post\u2010weaned piglets. Presumptive <jats:italic>Escherichia coli<\/jats:italic> isolates were confirmed by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u00a0<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>. Isolates (<jats:italic>n<\/jats:italic>\u00a0=\u00a0325) were screened for susceptibility to 19 veterinary antibiotics using <jats:styled-content style=\"fixed-case\">MIC<\/jats:styled-content> broth microdilution. All 325 <jats:italic>E.\u00a0coli<\/jats:italic> isolates underwent further testing against 27 antibiotics used in human medicine and were screened for <jats:styled-content style=\"fixed-case\">ETEC<\/jats:styled-content> adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>a, <jats:styled-content style=\"fixed-case\">ST<\/jats:styled-content>b, Stx2e and <jats:styled-content style=\"fixed-case\">LT<\/jats:styled-content>) by multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Isolates identified as phenotypically resistant to third\u2010generation cephalosporin (3<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) and aminoglycoside antibiotics were screened by multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\/reverse line blot to detect common \u03b2\u2010lactam and aminoglycosides resistance genes, confirmed by sequencing. Twenty (6.1%) of the <jats:italic>E.\u00a0coli<\/jats:italic> isolates were resistant to 3<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> antibiotics and 24 (7.4%) to the aminoglycoside antibiotic gentamicin. Genetic analysis revealed six different extended spectrum \u03b2\u2010lactamase (<jats:styled-content style=\"fixed-case\">ESBL<\/jats:styled-content>) genes (<jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\">CTX<\/jats:styled-content>\u2010M\u20101, \u201014, \u201015, \u201027, <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\">SHV<\/jats:styled-content>\u201012 and <jats:italic>bla<\/jats:italic><jats:styled-content style=\"fixed-case\">CMY<\/jats:styled-content>\u20102\u2010like genes), four of which have not been previously reported in Australian pigs. Critically, the prevalence of 3<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> resistance was higher in non\u2010pathogenic (non\u2010<jats:styled-content style=\"fixed-case\">ETEC<\/jats:styled-content>) isolates and those from clinically normal (non\u2010diarrhoeal) samples. This highlights the importance of non\u2010<jats:styled-content style=\"fixed-case\">ETEC<\/jats:styled-content><jats:italic>E.\u00a0coli<\/jats:italic> as reservoirs of antimicrobial resistance genes in piglet pens. Antimicrobial resistance surveillance in pig production focused on diagnostic specimens from clinically\u2010affected animals might be potentially misleading. We recommend that surveillance for emerging antimicrobial resistance such as to 3<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> antibiotics should include clinically healthy pigs.<\/jats:p>","DOI":"10.1111\/zph.12402","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T14:25:49Z","timestamp":1505744749000},"source":"Crossref","is-referenced-by-count":20,"title":["Antibiotic resistant <i>Escherichia coli<\/i> in southeastern Australian pig herds and implications for surveillance"],"prefix":"10.1111","volume":"65","author":[{"given":"L. K.","family":"van Breda","sequence":"first","affiliation":[{"name":"Sydney School of Veterinary Science The University of Sydney  Camden NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. P.","family":"Dhungyel","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science The University of Sydney  Camden NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9921-4986","authenticated-orcid":false,"given":"M. P.","family":"Ward","sequence":"additional","affiliation":[{"name":"Sydney School of Veterinary Science The University of Sydney  Camden NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2015.08.002"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02153-15"},{"key":"e_1_2_8_4_1","unstructured":"AURA(2016).First Australian report on antimicrobial use and resistance in human health \u2013 supplementary data 1\u201354. 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