{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T11:26:59Z","timestamp":1783423619788,"version":"3.54.6"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T00:00:00Z","timestamp":1510617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003046","name":"Empresa Brasileira de Pesquisa Agropecu\u00e1ria","doi-asserted-by":"publisher","award":["02.11.01.006"],"award-info":[{"award-number":["02.11.01.006"]}],"id":[{"id":"10.13039\/501100003046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["578102\/2008\u20100"],"award-info":[{"award-number":["578102\/2008\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>From 2009 to 2015, 74 lungs from suckling (6.8%), nursing (70.3%), fattening (20.3%) pigs and pregnant sows (2.7%) with respiratory signs from pig farms in Southern Brazil were submitted to a diagnostic laboratory for necropsy and\/or histologic examination and screening for respiratory agents by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>), virus isolation (<jats:styled-content style=\"fixed-case\">VI<\/jats:styled-content>) and subtyping for influenza A virus (<jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> and nested <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic> (Mhyo), <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for porcine circovirus 2 (<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>2), <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> for porcine reproductive and respiratory syndrome virus (<jats:styled-content style=\"fixed-case\">PRRSV<\/jats:styled-content>) and bacterial culture. All lung samples were positive for <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Seventy\u2010two lungs had histologic lesions associated with acute to subacute <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> infection characterized by necrotizing bronchiolitis\/bronchitis or bronchointerstitial pneumonia with lymphocytic peribronchiolitis and bronchiolar\/bronchial hyperplasia, respectively. Forty\u2010nine lungs (66.2%) were positive by <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> nucleoprotein. The H1N1\/2009 was the most common subtype and the only <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> detected in 58.1% of lungs, followed by H1N2 (9.5%) and H3N2 (6.8%). Coinfection of <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> and Mhyo was seen in 23 (31%) cases. Although 14.9% of the lungs were positive for <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>2 using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, no suggestive lesions of <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>2 disease were observed. Porcine reproductive and respiratory syndrome virus (<jats:styled-content style=\"fixed-case\">PRRSV<\/jats:styled-content>) was not detected, consistent with the <jats:styled-content style=\"fixed-case\">PRRS<\/jats:styled-content>\u2010free status of Brazil. Secondary bacterial infections (8\/38) were associated with suppurative bronchopneumonia and\/or pleuritis. Primary <jats:styled-content style=\"fixed-case\">IAV<\/jats:styled-content> infection with Mhyo coinfection was the most common agents found in porcine respiratory disease complex (<jats:styled-content style=\"fixed-case\">PRDC<\/jats:styled-content>) in pigs in Southern Brazil.<\/jats:p>","DOI":"10.1111\/zph.12424","type":"journal-article","created":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T04:46:51Z","timestamp":1510721211000},"source":"Crossref","is-referenced-by-count":30,"title":["Porcine respiratory disease complex after the introduction of H1N1\/2009 influenza virus in Brazil"],"prefix":"10.1111","volume":"65","author":[{"given":"R. R.","family":"Rech","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University  College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gava","sequence":"additional","affiliation":[{"name":"Embrapa Su\u00ednos e Aves  Conc\u00f3rdia SC Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Silva","sequence":"additional","affiliation":[{"name":"Qualem Laboratorio Veterin\u00e1rio  Santa Maria RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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