{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T12:10:02Z","timestamp":1751112602842,"version":"3.41.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T00:00:00Z","timestamp":1512691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001786","name":"University of Adelaide","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In Australia,<jats:italic>Salmonella<\/jats:italic>Typhimurium definitive type 9 is frequently isolated during foodborne outbreaks of salmonellosis. Multiple\u2010locus variable number tandem repeat analysis (<jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>) trace back investigations frequently identify isolate distribution patterns that may be epidemiologically linked to disease outbreaks. In this study, the in vitro virulence potential of<jats:italic>S<\/jats:italic>. Typhimurium<jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content>9 isolates possessing different<jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>patterns (03 15 07 11 550, 03 24 11 10 523, 03 15 08 11 550 and 03 14 08 11 550) isolated from either humans or layer hens was assessed using a human colon carcinoma cell line. Four strains per<jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>from each host for a total of 32 isolates were included in these experiments. Bacteria were grown to stationary phase and added to cells at a multiplicity of infection of 100. Across all isolates, mean percent recovery ranged from 7.1\u00a0\u00b1\u00a01.1 to 33.3\u00a0\u00b1\u00a07.1%. The layer hen isolate, KC900 (<jats:styled-content style=\"fixed-case\">MLVA<\/jats:styled-content>profile 03 15 08 11 550), exhibited the greatest invasion with a mean percent recovery of 33.3\u00a0\u00b1\u00a07.1%. Overall, layer hen isolates of S. Typhimurium DT9 had significantly higher invasion into Caco2 cells than human isolates (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0021).<jats:styled-content style=\"fixed-case\">RAPD<\/jats:styled-content>and enterobacterial repetitive intergenic consensus genomic fingerprinting was also performed. Irrespective of source, the<jats:italic>Salmonella<\/jats:italic><jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content>9 isolates included in this study exhibited similar fingerprint patterns.<\/jats:p>","DOI":"10.1111\/zph.12434","type":"journal-article","created":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T09:36:42Z","timestamp":1512725802000},"source":"Crossref","is-referenced-by-count":1,"title":["In vitro invasion capacity of<i>Salmonella<\/i>Typhimurium<scp>DT<\/scp>9 isolates sourced from humans and layer hen environments"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5793-1629","authenticated-orcid":false,"given":"A. R.","family":"McWhorter","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Phan","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Hocking","sequence":"additional","affiliation":[{"name":"Salmonella Reference Laboratory Microbiology and Infectious Diseases SA Pathology Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. K.","family":"Chousalkar","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R113.472274"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-007-9474-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00598-11"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1637\/7322-010705R.1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1637\/6098"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0787"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01041-13"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00954-10"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02931-14"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00090-10"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.33321\/cdi.2009.33.42","article-title":"Monitoring the incidence and causes of diseases potentially transmitted by food in Australia: Annual report of the OzFoodNet Network, 2008","volume":"33","author":"OzFoodNet Working Group","year":"2009","journal-title":"Communicable Diseases Intelligence"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"396","DOI":"10.33321\/cdi.2010.34.40","article-title":"Monitoring the incidence and causes of diseases potentially transmitted by food in Australia: Annual report of the OzFoodNet network, 2009","volume":"34","author":"OzFoodNet Working Group","year":"2010","journal-title":"Communicable Diseases Intelligence"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"E213","DOI":"10.33321\/cdi.2012.36.17","article-title":"Monitoring the incidence and causes of diseases potentially transmitted by food in Australia: Annual report of the ozfoodnet network","volume":"36","author":"OzFoodNet Working Group","year":"2012","journal-title":"Communicable Diseases Intelligence"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"E236","DOI":"10.33321\/cdi.2015.39.22","article-title":"Monitoring the incidence and causes of diseases potentially transmitted by food in Australia: Annual report of the OzFoodNet network, 2011","volume":"39","author":"OzFoodNet Working Group","year":"2015","journal-title":"Communicable Diseases Intelligence Quarterly Report"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00398.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-011-0719-4"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.044461-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2013.837862"},{"volume-title":"Annual complication of quarterly reports","year":"2012","author":"South Australian Salmonella Reference Laboratory","key":"e_1_2_8_21_1"},{"volume-title":"Annual complication of quarterly reports","year":"2013","author":"South Australian Salmonella Reference Laboratory","key":"e_1_2_8_22_1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933914000026"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01892.x"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fzph.12434","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.12434","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T11:33:19Z","timestamp":1751110399000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.12434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,8]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/zph.12434"],"URL":"https:\/\/doi.org\/10.1111\/zph.12434","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"type":"print","value":"1863-1959"},{"type":"electronic","value":"1863-2378"}],"subject":[],"published":{"date-parts":[[2017,12,8]]}}}