{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:10:11Z","timestamp":1773288611456,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP939\/12"],"award-info":[{"award-number":["PIP939\/12"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP365\/15"],"award-info":[{"award-number":["PIP365\/15"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT2666\/15"],"award-info":[{"award-number":["PICT2666\/15"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The serotype O113:H21 is considered one of the relevant non\u2010O157 STEC serotypes associated with severe human infections. Due to the increased detection of O113 strains and their relationship with clinical cases, which emphasizes the importance of this serogroup as an emerging pathogen, our aim was to determine the characteristics of STEC O113:H21 strains circulating in bovine cattle and retail meat from Argentina. For this purpose, we determined the presence and combinations of various virulence genes (and their variants) related to adhesion and toxicity in a collection of 34 isolates. Their genetic relatedness using multiple\u2010locus variable\u2010number tandem repeat analysis (MLVA) was also studied. Subtyping of <jats:italic>stx<\/jats:italic> genes indicated that O113:H21 strains circulating in Argentina mainly present <jats:italic>stx<\/jats:italic><jats:sub><jats:italic>2a<\/jats:italic><\/jats:sub> alone or together with <jats:italic>stx<\/jats:italic><jats:sub><jats:italic>2c<\/jats:italic><\/jats:sub> or, less frequent, with <jats:italic>stx<\/jats:italic><jats:sub><jats:italic>2d<\/jats:italic><\/jats:sub>, all of which are subtypes associated with human disease. We found plasmid markers, such as <jats:italic>saa, ehxA<\/jats:italic> and <jats:italic>subA,<\/jats:italic> in a higher proportion than previous studies, and five variants of <jats:italic>saa<\/jats:italic>, two of which were novel ones. In relation to MLVA subtyping, we detected a limited diversity among the isolates considering that several loci were not discriminative and, that in some farms, the same clone seemed to remain circulating throughout the year. The O113:H21 strains studied harbour several toxin and adhesion genes (<jats:italic>saa<\/jats:italic>,<jats:italic> espP, fimCD, ehaA, iha, hcpA, elfA, lpfO113, ecpA<\/jats:italic>,<jats:italic> subA<\/jats:italic>,<jats:italic> cdt\u2010V<\/jats:italic>) and Stx subtypes associated with human disease. Results also highlighted that subtyping of <jats:italic>stx<\/jats:italic> and <jats:italic>saa<\/jats:italic> is useful to discriminate O113:H21 strains that share virulence genes. In conclusion, this study shows that a number of O113:H21 strains that occur in foods and bovines could be pathogenic for humans. This situation calls for further attention in the prevention and control of foodborne disease caused by these strains.<\/jats:p>","DOI":"10.1111\/zph.12467","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T04:41:42Z","timestamp":1522039302000},"page":"569-577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular epidemiology of Shiga toxin\u2010producing O113:H21 isolates from cattle and meat"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7144-3907","authenticated-orcid":false,"given":"A. M.","family":"Sanso","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. V.","family":"Bustamante","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Cadona","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Alfaro","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E.","family":"C\u00e1ceres","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M. A.","family":"Lucchesi","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. L.","family":"Padola","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Facultad de Ciencias Veterinarias Centro de Investigaci\u00f3n Veterinaria de Tandil CONICET\u2010CIC\u2010UNCPBA  Tandil Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Alonso M. Z.(2012).Caracterizaci\u00f3n genot\u00edpica de Escherichia coli verocitotoxig\u00e9nico y Escherichia coli enteropatog\u00e9nico aisladas de pollo y sus productos. 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