{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:37:58Z","timestamp":1790318278078,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003751","name":"Ministerio de Sanidad, Servicios Sociales e Igualdad","doi-asserted-by":"publisher","award":["RD12\/0017\/0012"],"award-info":[{"award-number":["RD12\/0017\/0012"]}],"id":[{"id":"10.13039\/501100003751","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Diagnosis of acute hepatitis E virus (HEV) infection is established by detection of anti\u2010<jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> IgM antibodies by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> or by amplification of serum viral <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. Here, we evaluate the diagnostic value of testing <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> in saliva to identify patients with acute <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> infection. Prospective proof\u2010of\u2010concept study including patients with acute hepatitis. Whole blood and neat saliva samples were obtained from all patients. Saliva samples were processed and analysed for <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> within 2\u00a0hr after collection. A total of 34 patients with acute hepatitis and 12 healthy donors were included in the study. <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> in serum was confirmed by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in eight of these patients (23.5%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 12.2%\u201340.2%). <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> was isolated in the saliva of eight of 34 patients (23.5%; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 12.2%\u201340.2%). All patients with <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> amplified in saliva had detectable <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> in serum. <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> was isolated neither in the saliva of any of the 26 patients without detectable <jats:styled-content style=\"fixed-case\">HEV RNA<\/jats:styled-content> in serum nor in healthy donors. Our study suggests that acute <jats:styled-content style=\"fixed-case\">HEV<\/jats:styled-content> infection could be diagnosed by assessing viral load in saliva.<\/jats:p>","DOI":"10.1111\/zph.12472","type":"journal-article","created":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T15:01:24Z","timestamp":1523890884000},"page":"584-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Detection of hepatitis E virus <scp>RNA<\/scp> in saliva for diagnosis of acute infection"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5813-6889","authenticated-orcid":false,"given":"A.","family":"Rivero\u2010Juarez","sequence":"first","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Frias","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Lopez\u2010Lopez","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Martinez\u2010Peinado","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednico Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. \u00c1.","family":"Risalde","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Brieva","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Machuca","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1.","family":"Camacho","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Garc\u00eda\u2010Bocanegra","sequence":"additional","affiliation":[{"name":"Animal Health Department Veterinary Science College Universidad de C\u00f3rdoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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