{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:37:18Z","timestamp":1789900638781,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,27]],"date-time":"2019-01-27T00:00:00Z","timestamp":1548547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the present study was to detect DNA of <jats:italic>Toxoplasma gondii<\/jats:italic> in <jats:italic>Crassostrea<\/jats:italic> spp. oysters cultured in the state of Par\u00e1, Brazil. A total of 400 oysters were directly collected from a fixed rack system. Gills, gastrointestinal tract (GIT) and intervalvular liquid were separated and grouped into pool samples of 10 animals, resulting in 40 samples each of gills, GIT and intervalvular liquid. DNA extraction was performed using a commercial kit, and <jats:italic>T.\u00a0gondii<\/jats:italic> DNA was detected by nested PCR using the primers Toxo3 and Toxo4, which produced an amplification product of 155\u00a0bp of the <jats:italic>T.\u00a0gondii<\/jats:italic> gene B1. Nucleotide sequencing was performed for positive samples, and the obtained sequences were identified by comparison with sequences in GenBank. The DNA of <jats:italic>T.\u00a0gondii<\/jats:italic> was detected in 5.8% (7\/120) of the pool samples, of which 7.5% (3\/40) was in the GIT, 5% (2\/40) in the gills, and 5% (2\/40) was in the intervalvular liquid. The obtained sequences presented 100% identity and overlap with <jats:italic>T.\u00a0gondii<\/jats:italic> DNA sequences<jats:italic>.<\/jats:italic> This is the report of detection of <jats:italic>T.\u00a0gondii <\/jats:italic>DNA in oysters from genus <jats:italic>Crassostrea<\/jats:italic> spp. originating from the state of Par\u00e1, eastern Amazon.<\/jats:p>","DOI":"10.1111\/zph.12564","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T04:10:35Z","timestamp":1548648635000},"page":"296-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Detection of <i>Toxoplasma gondii<\/i> in <i>Crassostrea<\/i> spp. oysters cultured in an estuarine region in eastern Amazon"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-5316","authenticated-orcid":false,"given":"Thamillys R. 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T.","family":"Honorio","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Institute of Veterinary Medicine Federal University of Par\u00e1 (UFPA) Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo F. R.","family":"Melo","sequence":"additional","affiliation":[{"name":"Graduate Program in Residency in Veterinary Medicine Institute of Veterinary Medicine Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via N. L.","family":"Barros","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Parasitology, Graduate Program in Animal Health in the Amazon, Institute of Veterinary Medicine Federal University of Par\u00e1 (UFPA) Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Scofield","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Parasitology, Graduate Program in Animal Health in the Amazon, Institute of Veterinary Medicine Federal University of Par\u00e1 (UFPA) Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-7457","authenticated-orcid":false,"given":"Isis","family":"Abel","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Geoprocessing (EpiGeo), Graduate Program in Animal Health in the Amazon, Institute of Veterinary Medicine Federal University of Par\u00e1 (UFPA) Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8616-648X","authenticated-orcid":false,"given":"Carla C. 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