{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T07:10:25Z","timestamp":1780729825507,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study about <jats:italic>Leptospira<\/jats:italic>, particularly pathogenic strain, was conducted in the flood\u2010prone area in the Special Capital Region of Jakarta. The aim of this study was to discover and identify the serovars of pathogenic <jats:italic>Leptospira<\/jats:italic> in the environment, which might infect human during flooding. Seventy\u2010three samples, consisted of 36 samples of environmental water and 37 samples of soil, were collected from 5 districts of Jakarta. Their pH was measured, and the samples were then cultured in a modified Korthof's medium with 5\u2010fluorouracil (5\u2010FU) addition. Polymerase chain reaction, targeted on 23S rDNA (<jats:italic>rrl<\/jats:italic>), FlaB and LipL32 genes, was performed to identify <jats:italic>Leptospira<\/jats:italic> genus and differentiate the pathogenic. Identification of the pathogenic <jats:italic>Leptospira<\/jats:italic> was done utilising DNA sequencing. Seven samples showed amplification of <jats:italic>rrl\u2010<\/jats:italic>gene. <jats:italic>flaB<\/jats:italic> and <jats:italic>lipl32<\/jats:italic>\u2010PCR assay indicated one positive amplification band, each. Confirmation of <jats:italic>flaB<\/jats:italic> and <jats:italic>lipl32<\/jats:italic> amplicons by DNA sequencing and BLAST analysis showed <jats:italic>flaB<\/jats:italic> amplicon (G1B; GenBank accession number <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"MK006031\">MK006031.1<\/jats:ext-link>) had 94% similarity with <jats:italic>L.\u00a0licerasiae<\/jats:italic> (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"LC005426.1\">LC005426.1<\/jats:ext-link>), while <jats:italic>lipl32<\/jats:italic> amplicon was not identified as <jats:italic>lipl32<\/jats:italic> of <jats:italic>Leptospira<\/jats:italic>. Based on those results, one intermediate pathogenic and six saprophytic <jats:italic>Leptospira<\/jats:italic> were obtained from the environment in Jakarta.<\/jats:p>","DOI":"10.1111\/zph.12610","type":"journal-article","created":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T10:49:22Z","timestamp":1559904562000},"page":"597-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Leptospira<\/i> detection in flood\u2010prone environment of Jakarta, Indonesia"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6642-4864","authenticated-orcid":false,"given":"Dian","family":"Widiyanti","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine YARSI University Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Titiek","family":"Djannatun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine YARSI University Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ike Irmawati Purbo","family":"Astuti","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine YARSI University Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri Dian","family":"Maharsi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine YARSI University Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2060241"},{"issue":"3","key":"e_1_2_8_4_1","first-page":"123","article-title":"The survival of Leptospira interrogans serovar Hardjo in the Malaysian environment","volume":"3","author":"Bejo S. 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