{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T23:51:48Z","timestamp":1783641108621,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"North-South Cooperation, University of Zurich"},{"name":"Section of Epidemiology, University of Zurich"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leptospirosis is a zoonotic bacterial disease reported worldwide. In Uganda, seropositivity has been reported in both humans and domesticated animals, including cattle. However, it remains unknown whether cattle are shedding leptospires and thus acting as potential source for human leptospirosis. We conducted this cross\u2010sectional study in two cattle abattoirs in Kampala, Uganda between June and July 2017. Kidney and urine samples from 500 cattle sourced from across the country were analysed by real\u2010time PCR to establish the prevalence of <jats:italic>Leptospira<\/jats:italic>\u2010positive cattle and risk of exposure to abattoir workers. The species of infecting <jats:italic>Leptospira<\/jats:italic> was determined by amplification of <jats:italic>secY<\/jats:italic> gene and compared to reference sequences published in GenBank. Of 500 cattle tested, 36 (7.2%) had <jats:italic>Leptospira<\/jats:italic> DNA in their kidneys (carriers), 29 (5.8%) in their urine (shedders); with an overall prevalence (kidney and\/or urine) of 8.8%. <jats:italic>Leptospira borgpetersenii<\/jats:italic> was confirmed as the infecting species in three cattle and <jats:italic>Leptospira kirschneri<\/jats:italic> in one animal. Male versus female cattle (OR\u00a0=\u00a03, <jats:italic>p<\/jats:italic>\u2010value 0.003), exotic versus local breeds (OR\u00a0=\u00a021.3, <jats:italic>p<\/jats:italic>\u2010value 0.002) or cattle from Western Uganda (OR\u00a0=\u00a04.4, <jats:italic>p<\/jats:italic>\u2010value 0.001) and from regions across the border (OR\u00a0=\u00a03.3, <jats:italic>p<\/jats:italic>\u2010value 0.032) versus from the central region were more likely to be <jats:italic>Leptospira<\/jats:italic>\u2010positive. The daily risk of exposure of abattoir workers to \u22651 (kidney and\/or urine) positive carcass ranged from 27% (95% credibility interval 18.6\u201352.3) to 100% (95% CI 91.0\u2013100.0), with halal butchers and pluck inspectors being at highest risk. In conclusion, cattle slaughtered at abattoirs in Uganda carry and shed pathogenic <jats:italic>Leptospira<\/jats:italic> species; and this may pose occupation\u2010related risk of exposure among workers in these abattoirs, with workers who handle larger numbers of animals being at higher risk.<\/jats:p>","DOI":"10.1111\/zph.12616","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T05:09:22Z","timestamp":1561698562000},"page":"636-646","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Bovine leptospirosis in abattoirs in Uganda: Molecular detection and risk of exposure among workers"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5432-6407","authenticated-orcid":false,"given":"Lordrick","family":"Alinaitwe","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB) Makerere University Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clovice","family":"Kankya","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB) Makerere University Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn J.","family":"Allan","sequence":"additional","affiliation":[{"name":"Institute of Biodiversity, Animal Health and Comparative Medicine University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Rodriguez\u2010Campos","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty University of Bern Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4277-9983","authenticated-orcid":false,"given":"Paul","family":"Torgerson","sequence":"additional","affiliation":[{"name":"Section of Epidemiology, Vetsuisse Faculty University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anou","family":"Dreyfus","sequence":"additional","affiliation":[{"name":"Section of Epidemiology, Vetsuisse Faculty University of Zurich Zurich Switzerland"},{"name":"Department of Medicine Swiss Tropical and Public Health Institute Basel Switzerland"},{"name":"University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001374"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vetreccr-2017-000484"},{"key":"e_1_2_8_4_1","volume-title":"Leptospirosis in northern Tanzania: Exploring the role of rodents and ruminant livestock in a neglected public health problem","author":"Allan K. 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