{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:22:15Z","timestamp":1780636935614,"version":"3.54.1"},"reference-count":65,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T00:00:00Z","timestamp":1563148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000003","name":"ArcticNet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000003","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There are few epidemiologic studies on the role of dogs in zoonotic parasitic transmission in the Circumpolar North. The objectives of this study were to: (a) estimate the faecal prevalence of <jats:italic>Giardia<\/jats:italic> spp. and <jats:italic>Cryptosporidium<\/jats:italic> spp. in dogs; (b) investigate potential associations between the type of dog population and the faecal presence of <jats:italic>Giardia<\/jats:italic> spp. and <jats:italic>Cryptosporidium<\/jats:italic> spp.; and (c) describe the molecular characteristics of <jats:italic>Giardia<\/jats:italic> spp. and <jats:italic>Cryptosporidium<\/jats:italic> spp. in dogs in Iqaluit, Nunavut. We conducted two cross\u2010sectional studies in July and September 2016. In July, the team collected daily faecal samples for 3\u00a0days from each of 20 sled dogs. In September, the team collected three faecal samples from each of 59 sled dogs, 111 samples from shelter dogs and 104 from community dogs. We analysed faecal samples for the presence of <jats:italic>Giardia<\/jats:italic> spp. and <jats:italic>Cryptosporidium<\/jats:italic> spp. using rapid immunoassay and flotation techniques. Polymerase chain reaction (PCR) and sequencing of target genes were performed on positive faecal samples. Overall, the faecal prevalence of at least one of the target parasites, when one faecal sample was chosen at random for all dogs, was 8.16% (CI: 5.52\u201311.92), and for <jats:italic>Giardia<\/jats:italic> spp. and <jats:italic>Cryptosporidium<\/jats:italic> spp., prevalence was 4.42% (CI: 2.58\u20137.49) and 6.12% (CI: 3.88\u20139.53), respectively. The odds of faecal <jats:italic>Giardia<\/jats:italic> spp. in sled dogs were significantly higher than those in shelter and community dogs (OR 10.19 [CI: 1.16\u201389.35]). Sequence analysis revealed that 6 faecal samples were <jats:italic>Giardia intestinalis<\/jats:italic>, zoonotic assemblage B (<jats:italic>n<\/jats:italic>\u00a0=\u00a02) and species\u2010specific assemblages D (<jats:italic>n<\/jats:italic>\u00a0=\u00a03) and E (<jats:italic>n<\/jats:italic>\u00a0=\u00a01), and five faecal samples were <jats:italic>Cryptosporidium canis<\/jats:italic>. <jats:italic>Giardia intestinalis<\/jats:italic> is zoonotic; however, <jats:italic>Cryptosporidium canis<\/jats:italic> is rare in humans and, when present, usually occurs in immunosuppressed individuals. Dogs may be a potential source of zoonotic <jats:italic>Giardia intestinalis<\/jats:italic> assemblage B infections in residents in Iqaluit, Nunavut, Canada; however, the direction of transmission is unclear.<\/jats:p>","DOI":"10.1111\/zph.12628","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T11:09:14Z","timestamp":1563188954000},"page":"813-825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence and genetic characterization of <i>Giardia<\/i> spp. and <i>Cryptosporidium<\/i> spp. in dogs in Iqaluit, Nunavut, Canada"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3369-2088","authenticated-orcid":false,"given":"Danielle A.","family":"Julien","sequence":"first","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph Guelph Ontario Canada"},{"name":"Centre for Public Health and Zoonoses University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan M.","family":"Sargeant","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph Guelph Ontario Canada"},{"name":"Centre for Public Health and Zoonoses University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca A.","family":"Guy","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory at the Public Health Agency of Canada Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph Guelph Ontario Canada"},{"name":"Department of Pathology, Microbiology &amp; Immunology, School of Veterinary Medicine University of California Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel K.","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Ontario Veterinary College University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Bunce","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enooyaq","family":"Sudlovenick","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology Atlantic Veterinary College Charlottetown Prince Edward Island Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory Services Division University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiping","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory Services Division University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7298-8765","authenticated-orcid":false,"given":"Sherilee L.","family":"Harper","sequence":"additional","affiliation":[{"name":"Department of Population Medicine, Ontario Veterinary College University of Guelph Guelph Ontario Canada"},{"name":"Centre for Public Health and Zoonoses University of Guelph Guelph Ontario Canada"},{"name":"School of Public Health University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03403702"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-018-1386-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_10_5_1","volume-title":"Applied Biosystems DNA sequencing analysis software user guide","author":"Applied Biosystems","year":"2000"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.12.011"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.01.003"},{"issue":"10","key":"e_1_2_10_8_1","first-page":"1115","article-title":"Evaluation and delivery of domestic animal health services in remote communities in the Northwest Territories: A case study of status and needs","volume":"51","author":"Brook R. 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