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Definitive diagnosis is crucial for patients experiencing symptoms similar to other arboviral diseases because they can vary in clinical consequences. An increasing number of patients experience long\u2010term rheumatic effects of CHIKV infection, but these cases may not be optimally detected by molecular assays and anti\u2010CHIKV IgM ELISAs (M\u2010ELISAs) used for confirmation and screening, respectively. The subsequent confirmatory serological test, the plaque reduction neutralization test (PRNT), is laborious and time\u2010consuming. In this study, we evaluated a new diagnostic algorithm in which the M\u2010ELISA is conducted in parallel with an anti\u2010CHIKV IgG ELISA (G\u2010ELISA) and observed that the Euroimmun M\u2010ELISA combined with the Euroimmun G\u2010ELISA or the Abcam G\u2010ELISA exhibited excellent sensitivity and specificity for CHIKV. The combinations demonstrated perfect and near perfect inter\u2010rater agreement with the PRNT, respectively, suggesting their potential to be used as alternatives to the confirmatory serological PRNT assay for CHIKV.<\/jats:p>","DOI":"10.1111\/zph.12641","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T13:04:00Z","timestamp":1566824640000},"page":"909-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Combining anti\u2010IgM and IgG immunoassays for comprehensive chikungunya virus diagnostic testing"],"prefix":"10.1111","volume":"66","author":[{"given":"Emelissa J.","family":"Mendoza","sequence":"first","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssia","family":"Robinson","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Dimitrova","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Mueller","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Holloway","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Makowski","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4596-7349","authenticated-orcid":false,"given":"Heidi","family":"Wood","sequence":"additional","affiliation":[{"name":"Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory Public Health Agency of Canada Winnipeg MB Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)70160-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2110.141660"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/517537"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40384"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40383"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0719"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001446"},{"volume-title":"Mayaro virus disease \u2010 Canada: (AB) ex Peru","year":"2018","author":"Fonseca K.","key":"e_1_2_8_9_1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.909-932.2001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.01.011"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw274"},{"issue":"3","key":"e_1_2_8_13_1","first-page":"415","article-title":"Aedes furcifer and other mosquitoes as vectors of chikungunya virus at Mica, northeastern Transvaal, South Africa","volume":"6","author":"Jupp P. 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