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STEC virulence is considered to correlate with the amount of Shiga toxins (Stx) produced, especially Stx2, whose subtype Stx2a is most frequently associated with high virulence. Stx are encoded in prophages, which play an important role in STEC pathogenesis. The aim of this study was to evaluate <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub> expression levels and Stx2a phage production using qPCR and the double\u2010agar\u2010layer method in 29 STEC strains, corresponding to serotypes O26:H11 (6), O91:H21 (1), O145:H\u2010 (11) and O157:H7 (11), isolated from cattle and humans. Results were then tested for possible associations with serotype, origin or some genetic features. We observed heterogeneous levels of <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub> expression and Stx2a phage production. However, statistical comparisons identified a higher <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub> expression in response to mitomycin C in strains isolated from cattle than in those from humans. At the same time, compared to <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub>\/<jats:italic>stx<\/jats:italic><jats:sub>2c<\/jats:sub> strains, <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub> strains showed a higher increase in phage production under induced conditions. Notably, most of the strains studied, regardless of serotype and origin, carried inducible Stx2a phages and evidenced expression of <jats:italic>stx<\/jats:italic><jats:sub>2a<\/jats:sub> that increased along with phage production levels under induced conditions.<\/jats:p>","DOI":"10.1111\/zph.12653","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T12:14:13Z","timestamp":1577103253000},"page":"44-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparable <i>stx<\/i><sub>2a<\/sub> expression and phage production levels between Shiga toxin\u2010producing <i>Escherichia coli<\/i> strains from human and bovine origin"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9121-4570","authenticated-orcid":false,"given":"Julia","family":"Burg\u00e1n","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Centro de Investigaci\u00f3n Veterinaria de Tandil (CIVETAN) (CONICET\u2010CIC\u2010UNCPBA) Facultad de Ciencias Veterinarias Universidad Nacional del Centro de la Provincia de Buenos Aires Tandil Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1826-2143","authenticated-orcid":false,"given":"Alejandra","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Centro de Investigaci\u00f3n Veterinaria de Tandil (CIVETAN) (CONICET\u2010CIC\u2010UNCPBA) Facultad de Ciencias Veterinarias Universidad Nacional del Centro de la Provincia de Buenos Aires Tandil Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9014-3616","authenticated-orcid":false,"given":"Paula M. A.","family":"Lucchesi","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica y Biotecnolog\u00eda Centro de Investigaci\u00f3n Veterinaria de Tandil (CIVETAN) (CONICET\u2010CIC\u2010UNCPBA) Facultad de Ciencias Veterinarias Universidad Nacional del Centro de la Provincia de Buenos Aires Tandil Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ram.2016.04.009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.3718.1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00846-13"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03463-15"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.3.560-602.2004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/009704-0"},{"key":"e_1_2_8_8_1","unstructured":"Di Rienzo J. 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