{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:42:19Z","timestamp":1790034139135,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,22]],"date-time":"2019-12-22T00:00:00Z","timestamp":1576972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence of NDM\u2010producing <jats:italic>Escherichia coli<\/jats:italic> has considerably threatened human and animal health worldwide. This study describes for the first time in Egypt, the draft genome sequences of emerging NDM\u20105\u2010producing <jats:italic>E.\u00a0coli<\/jats:italic> from humans and dogs, and investigates genetic relatedness between isolates from both sources. Two <jats:italic>E.\u00a0coli<\/jats:italic> from human urine and seven from environmental clinical samples of dogs exhibited resistance to carbapenems and harbouring <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub> were subjected to Illumina Miseq whole\u2010genome sequencing (WGS). Assembly and analysis of the reads were performed to identify resistance genes, multilocus sequence types (MLST), plasmid replicon types (Inc) and insertion sequences (IS) of the <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub> region; core genome MLST (cgMLST) analysis was also performed. Two different NDM alleles were identified; <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20105<\/jats:sub> in <jats:italic>E.\u00a0coli<\/jats:italic> HR119 from the urine of a healthy person and environmental samples of dogs, and <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20101<\/jats:sub> in <jats:italic>E.\u00a0coli<\/jats:italic> HR135 from a human patient's urine. Multiple mobilizable resistance genes to different antimicrobial classes were identified except the colistin resistance gene, <jats:italic>mcr<\/jats:italic>. <jats:italic>E.\u00a0coli<\/jats:italic> isolates from humans and dogs were assigned to different sequence types (STs). Using cgMLST, dog isolates clustered together with only 1\u20132 allellic differences; however, human <jats:italic>E.\u00a0coli<\/jats:italic> showed 1,978 different allelles compared with dog isolates. Plasmidfinder results indicated the presence of an IncX3 replicon in <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20105<\/jats:sub>\u2010producing <jats:italic>E.\u00a0coli<\/jats:italic>; however, <jats:italic>bla<\/jats:italic><jats:sub>NDM\u20101<\/jats:sub> was linked to IncCoIKP3. Notably, the NDM region (3\u00a0Kb) in all isolates from humans and dogs was highly similar with variable flanking sequences that represented different IS elements. This study reports the first emergence of NDM\u20105\u2010producing <jats:italic>E.\u00a0coli<\/jats:italic> from dogs in Egypt that shared some genetic features with human isolates and could be considered potential public health threats.<\/jats:p>","DOI":"10.1111\/zph.12676","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T05:41:33Z","timestamp":1577079693000},"page":"324-329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Circulation of emerging NDM\u20105\u2010producing <i>Escherichia coli<\/i> among humans and dogs in Egypt"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6687-3758","authenticated-orcid":false,"given":"Hazem","family":"Ramadan","sequence":"first","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"},{"name":"Hygiene and Zoonoses Department Faculty of Veterinary Medicine Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushim K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Sharma","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Hygiene and Zoonoses Department Faculty of Veterinary Medicine Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lari M.","family":"Hiott","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Barrett","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffanie A.","family":"Woodley","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan G.","family":"Frye","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8480-4556","authenticated-orcid":false,"given":"Charlene R.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Bacterial Epidemiology and Antimicrobial Resistance Research Unit US National Poultry Research Center USDA\u2010ARS Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,22]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu661"},{"key":"e_1_2_9_4_1","volume-title":"Rapid risk assessment: Carbapenem\u2010resistant Enterobacteriaceae","author":"European Centre for Disease Prevention and 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