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Dog and pig ownership is associated with human infection, although the mechanism through which animals increase risk remains unknown. We first confirmed this association in Kintampo North, Ghana, using a retrospective analysis and serology, followed by a prospective molecular study of animal faeces. As a proxy of exposure to dog faeces, we analysed immunoreactivity of human serum to the zoonotic nematode <jats:italic>Toxocara canis<\/jats:italic>. Anti\u2010<jats:italic>Toxocara<\/jats:italic> antibodies were present in 62% of samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a089), and reactivity was associated with dog ownership. A subsequent prospective study revealed that 43% of dog and 56% of pig faecal samples contained hookworm eggs by microscopy. PCR analysis confirmed the presence of <jats:italic>N.\u00a0americanus<\/jats:italic> DNA in 47% of samples from dogs and 56% pig samples. Nematode larvae were successfully cultured from samples collected from 36 dogs and seven pigs. These results demonstrate that dogs and pigs have a likely role in the transmission of <jats:italic>N.\u00a0americanus<\/jats:italic> in endemic communities.<\/jats:p>","DOI":"10.1111\/zph.12708","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T06:23:15Z","timestamp":1591942995000},"page":"474-483","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Dogs and pigs are transport hosts of <i>Necator americanus<\/i>: Molecular evidence for a zoonotic mechanism of human hookworm transmission in Ghana"],"prefix":"10.1111","volume":"67","author":[{"given":"Ryan H.","family":"Boyko","sequence":"first","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Marie Harrison","sequence":"additional","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie","family":"Humphries","sequence":"additional","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison P.","family":"Galvani","sequence":"additional","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey P.","family":"Townsend","sequence":"additional","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Otchere","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research University of Ghana Legon Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Noguchi Memorial Institute for Medical Research University of Ghana Legon Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2255-8833","authenticated-orcid":false,"given":"Michael","family":"Cappello","sequence":"additional","affiliation":[{"name":"Yale School of Medicine New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118524"},{"issue":"1","key":"e_1_2_8_3_1","first-page":"1","article-title":"Investigations on the control of hookworm disease. 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