{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:47:49Z","timestamp":1790981269346,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T00:00:00Z","timestamp":1594339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["106698\/Z\/14\/Z"],"award-info":[{"award-number":["106698\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Arthropod\u2010borne diseases are widespread worldwide and are a complex interaction between animals, humans and ectoparasites. The understanding of the diversity and epidemiology of organisms transmitted by arthropod vectors, and the role of hosts and vectors in transmission of infections remain limited in Lao PDR. What knowledge does exist is primarily focused on more rural regions of the country. This study screened ectoparasites from domestic dogs in Vientiane city for the presence of bacterial pathogens of zoonotic importance. A total of 3,511 arthropod vectors were collected from 112 dogs. Vectors collected were\n                    <jats:italic>Rhipicephalus sanguineus<\/jats:italic>\n                    ticks,\n                    <jats:italic>Ctenocephalides felis felis and Ctenocephalides felis orientis<\/jats:italic>\n                    fleas and\n                    <jats:italic>Heterodoxus spiniger<\/jats:italic>\n                    lice. A sub\u2010sample of vectors from each dog was analysed by PCR to identify the potential bacteria. From 129 vector pools,\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp. was detected in 6.7% (7\/105) pools of ticks, 86.4% (19\/22) pools of fleas and both pools of lice. Sequencing analysis confirmed\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    in 13 flea pools and one louse pool and\n                    <jats:italic>Rickettsia asembonensis<\/jats:italic>\n                    in six flea pools. Anaplasmataceae was identified in 14.3% (15\/105) tick pools and 100% (22\/22) flea pools. Sequencing revealed the presence of\n                    <jats:italic>Anaplasma platys<\/jats:italic>\n                    in ticks and\n                    <jats:italic>Wolbachia pipientis<\/jats:italic>\n                    in fleas.\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. was detected in one tick and one louse pool, and\n                    <jats:italic>Brucella<\/jats:italic>\n                    spp. was detected in 12.4% (13\/105) tick pools. All samples were negative for\n                    <jats:italic>Bartonella<\/jats:italic>\n                    spp.,\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    and\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    . This is the first study providing evidence of\n                    <jats:italic>R.\u00a0asembonensis<\/jats:italic>\n                    in fleas in Laos. Results from this study show arthropods are potential vectors to transmit zoonotic infection in Vientiane city, suggesting humans are at risk of zoonotic infections in the city.\n                  <\/jats:p>","DOI":"10.1111\/zph.12753","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T11:49:05Z","timestamp":1594381745000},"page":"862-868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Screening of ectoparasites from domesticated dogs for bacterial pathogens in Vientiane, Lao PDR"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5325-8329","authenticated-orcid":false,"given":"Hung Manh","family":"Nguyen","sequence":"first","affiliation":[{"name":"Lao Tropical and Public Health Institute Vientiane Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Watthana","family":"Theppannga","sequence":"additional","affiliation":[{"name":"National Animal Health Laboratories Vientiane Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khamsing","family":"Vongphayloth","sequence":"additional","affiliation":[{"name":"Institut Pasteur du Laos Vientiane Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bounlom","family":"Douangngeun","sequence":"additional","affiliation":[{"name":"National Animal Health Laboratories Vientiane Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Blacksell","sequence":"additional","affiliation":[{"name":"Mahidol\u2010Oxford Tropical Medicine Research Unit Bangkok Thailand"},{"name":"Centre for Tropical Medicine, Nuffield Department of Medicine University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1187-2712","authenticated-orcid":false,"given":"Matthew T.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Centre for Tropical Medicine, Nuffield Department of Medicine University of Oxford Oxford UK"},{"name":"Lao\u2010Oxford\u2010Mahosot Hospital\u2010Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory Mahosot Hospital Vientiane Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0372"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2012.03.015"},{"key":"e_1_2_9_4_1","first-page":"77","article-title":"Insectes ectoparasites du chien au Laos","volume":"152","author":"Beaucournu J. 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