{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T14:35:46Z","timestamp":1774449346505,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T00:00:00Z","timestamp":1607212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Murine typhus, a neglected rickettsiosis caused by <jats:italic>Rickettsia typhi<\/jats:italic>, is a common disease in several Latin\u2010American countries. The sylvatic life cycle of <jats:italic>R.\u00a0typhi<\/jats:italic> encompasses the presence of several wild mammals, particularly opossums of the genus <jats:italic>Didelphis<\/jats:italic> and their associated fleas. Due to the colonization of wild environments by human populations, the increase in contact with opossum fleas has generated the presence of urban outbreaks of typhus. For this reason, the aim of our study was to identify the presence and diversity of <jats:italic>Rickettsia<\/jats:italic> sp. in fleas collected from opossums of an urban reserve in Mexico City. Opossums were captured from February to September 2017. For the detection of <jats:italic>Rickettsia<\/jats:italic> DNA, fragments of 800\u00a0bp of the citrate synthase (<jats:italic>gltA<\/jats:italic>) and the outer membrane protein B (<jats:italic>ompB<\/jats:italic>) were amplified. A total of 141 fleas (111 \u2640, 30 \u2642) of a single species (<jats:italic>Ctenocephalides felis felis<\/jats:italic>) were recovered from 31 <jats:italic>Didelphis virginiana<\/jats:italic>. <jats:italic>Rickettsia<\/jats:italic> DNA was detected in 17.7% (25\/141) of the analysed fleas, recovered from seven infested opossums. The Maximum likelihood of sequences exhibited an identity of 99%\u2013100% with sequences of <jats:italic>R.\u00a0typhi<\/jats:italic> from southern United States. This work represents the first record of <jats:italic>R.\u00a0typhi<\/jats:italic> in fleas from opossums in Mexico.<\/jats:p>","DOI":"10.1111\/zph.12770","type":"journal-article","created":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T08:16:16Z","timestamp":1607242576000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Urban foci of murine typhus involving cat fleas (<i>Ctenocephalides felis felis<\/i>) collected from opossums in Mexico City"],"prefix":"10.1111","volume":"68","author":[{"given":"Ver\u00f3nica","family":"Santoyo\u2010Col\u00edn","sequence":"first","affiliation":[{"name":"Departamento de Producci\u00f3n Agr\u00edcola y Animal Universidad Aut\u00f3noma Metropolitana \u2013Unidad Xochimilco Ciudad de M\u00e9xico 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M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7935-9758","authenticated-orcid":false,"given":"Her\u00f3n","family":"Huerta\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda Instituto de Diagn\u00f3stico y Referencia Epidemiol\u00f3gicos Secretar\u00eda de Salud Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Alc\u00e1ntara\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Unidad Departamental de Vigilancia Epidemiol\u00f3gica de la Red de Hospitales de la Ciudad de M\u00e9xico Secretar\u00eda de Salud del Gobierno de la Ciudad de M\u00e9xico Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3821-4998","authenticated-orcid":false,"given":"Ingeborg","family":"Becker","sequence":"additional","affiliation":[{"name":"Centro de Medicina Tropical Divisi\u00f3n de Investigaci\u00f3n Facultad de Medicina Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3956-4415","authenticated-orcid":false,"given":"Fernando","family":"Gual\u2010Sill","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Agr\u00edcola y Animal Universidad Aut\u00f3noma Metropolitana \u2013Unidad Xochimilco Ciudad de M\u00e9xico M\u00e9xico"},{"name":"Direcci\u00f3n General de Zool\u00f3gicos y Conservaci\u00f3n de la Fauna Silvestre Secretar\u00eda del Medio Ambiente de la Ciudad de M\u00e9xico Ciudad de M\u00e9xico M\u00e9xico"},{"name":"Departamento de Etolog\u00eda Fauna Silvestre y Animales de Laboratorio Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5726-7943","authenticated-orcid":false,"given":"Andr\u00e9s M.","family":"L\u00f3pez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Etolog\u00eda Fauna Silvestre y Animales de Laboratorio Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad de M\u00e9xico M\u00e9xico"},{"name":"School of Veterinary Medicine Department of Medicine and Epidemiology University of California Davis CA 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