{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:12:20Z","timestamp":1780452740637,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this work was the establishment of a national laboratory sentinel surveillance service for human clinical <jats:italic>Campylobacter<\/jats:italic> in Ireland. This included detailed genomic molecular epidemiology of <jats:italic>Campylobacter<\/jats:italic> for 2019. For February\u2010December 2019, 24 clinical microbiology laboratories in Ireland submitted all PCR\/culture\u2010positive clinical <jats:italic>Campylobacter<\/jats:italic> spp. specimens to Public Health Laboratory (PHL) Dublin one\u00a0week out of every four. Antimicrobial susceptibility testing (AST) according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria was carried out for <jats:italic>Campylobacter<\/jats:italic> spp. isolates for ciprofloxacin, tetracycline and erythromycin. Batch whole genome sequencing (WGS) was carried out on cultures and analysis was performed to determine species, genotype, identify antimicrobial resistance (AMR) and virulence determinants and identify clusters. A total of 75 isolates and 366 PCR\u2010positive stools were received, and 277 isolates recovered (55.7% recovery from stools). Of 257 isolates characterized by WGS, 86.4% (<jats:italic>n<\/jats:italic>\u00a0=\u00a0222) were <jats:italic>Campylobacter jejuni<\/jats:italic>, 11.7% (<jats:italic>n<\/jats:italic>\u00a0=\u00a030) <jats:italic>Campylobacter coli<\/jats:italic> and 1.9% (<jats:italic>n<\/jats:italic>\u00a0=\u00a05) <jats:italic>Campylobacter lari<\/jats:italic>. There were 20 clonal complexes with ST\u201021 clonal complex most prevalent at 26.8% (<jats:italic>n<\/jats:italic>\u00a0=\u00a069). 50.5% (<jats:italic>n<\/jats:italic>\u00a0=\u00a0140) of isolates were susceptible to all three antimicrobials tested. 39.3% (<jats:italic>n<\/jats:italic>\u00a0=\u00a0109) isolates were ciprofloxacin resistant, 26.3% (<jats:italic>n<\/jats:italic>\u00a0=\u00a073) tetracycline resistant and two isolates erythromycin resistant. Congruence between phenotypic and genotypic AST was observed. There was 95.9% and 95.6% sensitivity and specificity for WGS to predict ciprofloxacin sensitivity and 98.6% and 99.5% sensitivity and specificity for WGS to predict tetracycline sensitivity. Virulence factors <jats:italic>flaA<\/jats:italic>, <jats:italic>racR<\/jats:italic>, <jats:italic>ciaB<\/jats:italic> and <jats:italic>cdtB<\/jats:italic> were detected in all isolates. WGS identified 31 potential clusters for public health alert. This sentinel surveillance of human campylobacteriosis in Ireland establishes the basis for a national reference service. Linking with other partners in a \u2018One Health\u2019 framework will help us better understand sources of infection to reduce disease burden and the threat of AMR.<\/jats:p>","DOI":"10.1111\/zph.12802","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T06:11:52Z","timestamp":1610691112000},"page":"121-130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Establishment of sentinel surveillance of human clinical campylobacteriosis in Ireland"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2967-2762","authenticated-orcid":false,"given":"Carina","family":"Brehony","sequence":"first","affiliation":[{"name":"Public Health Laboratory Health Service Executive Dublin Ireland"},{"name":"European Public Health Microbiology Training Programme (EUPHEM) European Centre for Disease Control and Prevention Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donal","family":"Lanigan","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Health Service Executive Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Carroll","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Health Service Executive Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor","family":"McNamara","sequence":"additional","affiliation":[{"name":"Public Health Laboratory Health Service Executive Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.11.020"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66771-7"},{"issue":"266","key":"e_1_2_8_5_1","first-page":"30130","article-title":"The recurrent campylobacteriosis epidemic over Christmas and New Year in European countries, 2006\u20132014","volume":"10","author":"Bless P. 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