{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T02:08:24Z","timestamp":1774490904540,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Escherichia albertii<\/jats:italic>, often misidentified as <jats:italic>Escherichia coli<\/jats:italic>, has become an emerging foodborne human enteric pathogen. However, the prevalence and major animal reservoirs of this significant pathogen are still not clear. Here, we performed comprehensive microbiological, molecular, comparative genomics and animal studies to understand the status and features of <jats:italic>E. albertii<\/jats:italic> in the US domestic and food animals. Although no <jats:italic>E. albertii<\/jats:italic> was identified in a total of 1,022 diverse <jats:italic>E. coli<\/jats:italic> strains isolated from pets and food animals in a retrospective screening, in a pilot study, <jats:italic>E. albertii<\/jats:italic> was successfully isolated from a broiler farm (6 out of 20 chickens). The chicken <jats:italic>E. albertii<\/jats:italic> isolates showed clonal relationship as indicated by both pulsed\u2010field gel electrophoresis (PFGE) and whole\u2010genome sequence analysis. The isolated chicken <jats:italic>E. albertii<\/jats:italic> displayed multidrug resistance; all the resistance determinants including the extended\u2010spectrum beta\u2010lactamase gene, carried by plasmids, could be conjugatively transferred to <jats:italic>E. coli<\/jats:italic>, which was further confirmed by S1\u2010PFGE and Southern hybridization. Whole\u2010genome sequence\u2010based phylogenetic analysis showed the chicken <jats:italic>E. albertii<\/jats:italic> strains were phylogenetically close to those of human origins. Challenge experiment demonstrated that the <jats:italic>E. albertii<\/jats:italic> strains isolated from human and wild bird could successfully colonize in the chicken intestine. Together, this study, for the first time, reported the isolation of <jats:italic>E. albertii<\/jats:italic> in poultry at the pre\u2010hrvest level. The findings from multi\u2010tier characterization of the chicken <jats:italic>E. albertii<\/jats:italic> strains indicated the importance of chickens as a reservoir for <jats:italic>E. albertii<\/jats:italic>. A large scale of <jats:italic>E. albertii<\/jats:italic> survey in poultry production at the pre\u2010harvest level is highly warranted in the future.<\/jats:p>","DOI":"10.1111\/zph.12812","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T15:57:59Z","timestamp":1612454279000},"page":"213-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Isolation and characterization of <i>Escherichia albertii<\/i> in poultry at the pre\u2010harvest level"],"prefix":"10.1111","volume":"68","author":[{"given":"Atsushi","family":"Hinenoya","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville TN USA"},{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"},{"name":"Asian Health Science Institute Osaka Prefecture University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing\u2010Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville TN USA"},{"name":"College of Animal Science and Technology Henan University of Science and Technology Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2795-6988","authenticated-orcid":false,"given":"Ximin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orhan","family":"Sahin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine Iowa State University Ames IA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney A.","family":"Moxley","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences University of Nebraska\u2010Lincoln Lincoln NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine M.","family":"Logue","sequence":"additional","affiliation":[{"name":"Department of Population Health College of Veterinary Medicine University of Georgia Athens GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Graduate School of Life and Environmental Sciences Osaka Prefecture University Osaka Japan"},{"name":"Asian Health Science Institute Osaka Prefecture University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0377-1030","authenticated-orcid":false,"given":"Jun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.4.1507-1513.1991"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_2_8_4_1","volume-title":"Babraham Bioinformatics","author":"Andrews S.","year":"2010"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00254-18"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03378-14"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00004-14"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2011.09.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2018.1558961"},{"key":"e_1_2_8_14_1","volume-title":"Reservoirs of infection: The epidemiological characteristics of an emerging Pathogen, Escherichia albertii","author":"Gordon D. 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