{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:39Z","timestamp":1787016219268,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T00:00:00Z","timestamp":1613260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Enterohepatic\n                    <jats:italic>Helicobacter<\/jats:italic>\n                    (EHH) species have been increasingly associated with acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases in humans. However, their host range and transmission routes are poorly understood. Therefore, the aim of this study was to determine the presence of EHH in healthy dogs using both cultivation\u2010dependent and \u2010independent methods. Three hundred and ninety faecal samples from domestic dogs without gastrointestinal symptoms were analysed between June 2018 and July 2019 in Valdivia (South of Chile). Samples were inoculated on selective medium and in parallel were filtrated over an antibiotic\u2010free blood agar. Both media were incubated in a microaerobic atmosphere at 37\u00b0C for 7\u00a0days. Colonies were identified by PCR and phylogenetic analysis. A subset of 50 samples (half of them positive for EHH by cultivation and the remaining half negative) was analysed by PCR\u2010Denaturing Gradient Gel Electrophoresis (PCR\u2010DGGE) for direct detection. Cultivation method detected EHH in 15.4% (60\/390) of the samples, being the most prevalent species\n                    <jats:italic>H. canis<\/jats:italic>\n                    (5.8%, 23\/390) and\n                    <jats:italic>H. canicola<\/jats:italic>\n                    (5.1%, 20\/390), followed by\n                    <jats:italic>H. bilis<\/jats:italic>\n                    (3.6%, 14\/390) and \u2018H. winghamensis\u2019 (1.3%, 5\/390). In contrast, PCR\u2010DGGE method detected\n                    <jats:italic>Helicobacter<\/jats:italic>\n                    DNA in almost all (96%, 48\/50) tested samples. On the other hand, the method used also allowed to isolate other Campylobacterales, in fact 44.3% (173\/390) of the samples were positive for\n                    <jats:italic>Campylobacter upsaliensis<\/jats:italic>\n                    (43.3%, 169\/390) followed by\n                    <jats:italic>C. jejuni<\/jats:italic>\n                    (2.0%, 8\/390). Moreover, two strains that presented\n                    <jats:italic>Campylobacter<\/jats:italic>\n                    \u2010like morphology were finally identified as\n                    <jats:italic>Anaerobiospirillum succiniciproducens<\/jats:italic>\n                    . Our results indicate that healthy domestic dogs commonly carry EHH and other\n                    <jats:italic>Campylobacter<\/jats:italic>\n                    species. However, further studies are needed to determine whether and how these\n                    <jats:italic>Helicobacter<\/jats:italic>\n                    and\n                    <jats:italic>Campylobacter<\/jats:italic>\n                    species can be transmitted to humans.\n                  <\/jats:p>","DOI":"10.1111\/zph.12817","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T20:37:08Z","timestamp":1613335028000},"page":"344-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exploring the role of healthy dogs as hosts of enterohepatic\n                    <i>Helicobacter<\/i>\n                    species using cultivation\u2010dependent and \u2010independent approaches"],"prefix":"10.1111","volume":"68","author":[{"given":"Sof\u00eda","family":"Ochoa","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Microbiology Faculty of Sciences Universidad Austral de Chile Valdivia Chile"},{"name":"ANID \u2010 Millennium Science Initiative Program \u2010 Millennium Nucleus in the Biology of the Intestinal Microbiota Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Ojeda","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences Faculty of Veterinary Sciences Universidad Austral de Chile Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar A.","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Microbiology Faculty of Sciences Universidad Austral de Chile Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Vidal\u2010Veuthey","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Microbiology Faculty of Sciences Universidad Austral de Chile Valdivia Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7140-4219","authenticated-orcid":false,"given":"Luis","family":"Collado","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Microbiology Faculty of Sciences Universidad Austral de Chile Valdivia Chile"},{"name":"ANID \u2010 Millennium Science Initiative Program \u2010 Millennium Nucleus in the Biology of the Intestinal Microbiota Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02548-12"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2018.1475268"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-009-9067-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05314-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638718820082"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.07.035"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.7.1916-1917.1993"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.03.026"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-011-9931-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2016.02.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-5-1781"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.17.6106-6118.2005"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.05.001"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01351-16"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.004622-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2015.03.001"},{"key":"e_1_2_9_18_1","first-page":"233","volume-title":"Non\u2010Helicobacter pylori Helicobacter Infections in Humans and Animals. 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