{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T20:12:53Z","timestamp":1787083973668,"version":"build-2736575974"},"reference-count":93,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["11170363"],"award-info":[{"award-number":["11170363"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Shiga toxin\u2010producing <jats:italic>Escherichia coli<\/jats:italic> (STEC) is a zoonotic pathogen that causes severe illness in humans, often associated with foodborne outbreaks. Antimicrobial resistance among foodborne <jats:italic>E.\u00a0coli<\/jats:italic> has increased over the last decades becoming a public health issue. In this study, the presence and features of STEC were investigated in samples of meat, seafood, vegetables and ready\u2010to\u2010eat street\u2010vended food collected in Chile, using a genomic and microbiological approach. Phenotypic and genotypic antimicrobial resistance profiles were determined, and serotype, phylogroup, sequence type (ST) and phylogenomics were predicted using bioinformatic tools. Three thousand three hundred samples collected in 2019 were screened, of which 18 were positive for STEC strains (0.5%), with <jats:italic>stx<\/jats:italic>2a (61.1%) being the predominant <jats:italic>stx<\/jats:italic> subtype. The presence of the virulence genes <jats:italic>lpf<\/jats:italic>A (100%), <jats:italic>iha<\/jats:italic> and <jats:italic>eha<\/jats:italic>A (94.4%), and <jats:italic>ehx<\/jats:italic>A, <jats:italic>hly<\/jats:italic>A and <jats:italic>saa<\/jats:italic> (83.3%) was confirmed among the STEC strains; the Locus of adhesion and autoaggregation (LAA) was predicted in 14 (77.8%) strains. Strains displayed resistance to colistin (100%), and intermediate resistance to enrofloxacin (11.1%) and chloramphenicol (5.6%). In this regard, mutations in the two\u2010component regulatory system genes <jats:italic>pmr<\/jats:italic>A (S29G), <jats:italic>pmr<\/jats:italic>B (D283G) and <jats:italic>pho<\/jats:italic>P (I44L), and the presence of the <jats:italic>qnr<\/jats:italic>B19 gene were confirmed. STEC strains belonged to ST11231 (38.9%), ST297 and ST58 (16.7% each), and ST1635, ST11232, ST446, ST442 and ST54 (5.6% each), and the most frequently detected serotypes were O113:H21 (44.4%), O130:H11 and O116:H21 (16.7% each), and O174:H21 (11.1%). Strains belonging to the international ST58 showed genomic relatedness with worldwide strains from human and non\u2010human sources. Our study reports for the first time the genomic profile of STEC strains isolated from food in Chile, highlighting the presence of international clones and sequence types commonly associated with human infections in different geographical regions, as well as the convergence of virulence and resistance in STEC lineages circulating in this country.<\/jats:p>","DOI":"10.1111\/zph.12818","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T04:39:55Z","timestamp":1614055195000},"page":"226-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Genomic features and antimicrobial resistance patterns of Shiga toxin\u2010producing <i>Escherichia coli<\/i> strains isolated from food in Chile"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5923-6219","authenticated-orcid":false,"given":"Fernando","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Veterinarias y Pecuarias Departamento de Medicina Preventiva Animal Universidad de Chile Santiago Chile"},{"name":"Programa de Mag\u00edster en Ciencias Animales y Veterinarias Facultad de Ciencias Veterinarias y Pecuarias Universidad de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8913-2787","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Fuenzalida","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias y Pecuarias Departamento de Medicina Preventiva Animal Universidad de Chile Santiago 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