{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:38:09Z","timestamp":1791686289149,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007652","name":"Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria","doi-asserted-by":"publisher","award":["RTA2017\u201000055\u2010C02\u201001"],"award-info":[{"award-number":["RTA2017\u201000055\u2010C02\u201001"]}],"id":[{"id":"10.13039\/100007652","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Real\u2010time PCR analysis of environmental samples (dust and aerosols) is an easy tool to investigate the presence of\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    in the farm environment. The aim of this study was to assess the distribution of\n                    <jats:italic>C.\u00a0burnetii<\/jats:italic>\n                    DNA in dust collected inside animal premises from 272 small ruminant farms in Bizkaia (northern Spain), a region with recent reports of human Q fever cases and outbreaks. Within each farm, 5 samples of dust were collected from difference surfaces, and data on animal census, management procedures, characteristics of the premises and geographic location were collected. Real\u2010time PCR analysis of the dust samples detected presence of\n                    <jats:italic>C.\u00a0burnetii<\/jats:italic>\n                    DNA in 98 farms (36.0%), flock\u2010prevalence being higher in sheep (38.9%) or mixed ovine\u2010caprine production systems (36.8%), compared to goats (25.0%). Larger bacterial burdens were observed in mixed farms, compared to sheep (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.05). Single nucleotide polymorphism (SNP) analysis identified 5 different genotypes, with SNP8 being the predominant genotype (73%), followed by SNP6 (11%), SNP2 (9%), SNP4 (5%) and SNP1 (2%). Proportion of farms where\n                    <jats:italic>C.\u00a0burnetii<\/jats:italic>\n                    DNA was detected differed among the different agricultural counties, and a higher proportion of\n                    <jats:italic>C.\u00a0burnetii<\/jats:italic>\n                    DNA positive farms was associated with the occurrence of recent human Q fever outbreaks at several geographical locations. Dust sampling in domestic ruminant farms coupled with real\u2010time PCR to screen for the presence of\n                    <jats:italic>C.\u00a0burnetii<\/jats:italic>\n                    and estimate bacterial load can be a useful tool to identify herds and regions with high prevalence, define priority actions and monitor the effect of control measures. If combined with molecular genotyping and spatial distribution maps, it can help to identify farm contamination sources and trace the origin of human outbreaks.\n                  <\/jats:p>","DOI":"10.1111\/zph.12871","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T20:49:30Z","timestamp":1625777370000},"page":"666-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Analysis of environmental dust in goat and sheep farms to assess\n                    <i>Coxiella burnetii<\/i>\n                    infection in a Q fever endemic area: Geographical distribution, relationship with human cases and genotypes"],"prefix":"10.1111","volume":"68","author":[{"given":"Ion I.","family":"Zendoia","sequence":"first","affiliation":[{"name":"Department of Animal Health NEIKER\u2010Basque Institute for Agricultural Research and Development Basque Research and Technology Alliance (BRTA) Derio Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas F.","family":"Barandika","sequence":"additional","affiliation":[{"name":"Department of Animal Health NEIKER\u2010Basque Institute for Agricultural Research and Development Basque Research and Technology Alliance (BRTA) Derio Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Hurtado","sequence":"additional","affiliation":[{"name":"Department of Animal Health NEIKER\u2010Basque Institute for Agricultural Research and Development Basque Research and Technology Alliance (BRTA) Derio Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ceferino M.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology Veterinary Faculty University of Santiago de Compostela Lugo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Alonso","sequence":"additional","affiliation":[{"name":"Departamento de Salud del Gobierno Vasco Subdirecci\u00f3n de Salud P\u00fablica de Bizkaia Unidad de Vigilancia Epidemiol\u00f3gica Bilbao Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xabier","family":"Beraza","sequence":"additional","affiliation":[{"name":"Departamento de Salud del Gobierno Vasco Subdirecci\u00f3n de Salud P\u00fablica de Bizkaia Unidad de Vigilancia Epidemiol\u00f3gica Bilbao Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca","family":"Ocabo","sequence":"additional","affiliation":[{"name":"Departamento de Agricultura Servicio de Ganader\u00eda Diputaci\u00f3n Foral de Bizkaia Bilbao Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7404-1380","authenticated-orcid":false,"given":"Ana L.","family":"Garc\u00eda\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Animal Health NEIKER\u2010Basque Institute for Agricultural Research and Development Basque Research and Technology Alliance (BRTA) Derio Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12791"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006338"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0225605"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138817"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13028-018-0429-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01650-18"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00352"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.247.12.1379"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.04.010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05530-11"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268819002115"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9080652"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.01.025"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.11.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.07.011"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13137"},{"issue":"37","key":"e_1_2_9_18_1","first-page":"18975","article-title":"Q fever in the Netherlands\u2010what matters is seriousness of disease rather than quantity","volume":"13","author":"Cilla G.","year":"2008","journal-title":"Euro Surveillance"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3135-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02799-15"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12163"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07323-11"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151281"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/136946"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2010.1595"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00045-16"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.12778"},{"key":"e_1_2_9_28_1","volume-title":"Efectivos y producciones ganaderas de caprino","author":"Gobierno Vasco","year":"2020"},{"key":"e_1_2_9_29_1","volume-title":"Efectivos y producciones ganaderas\u2010ovino","author":"Gobierno Vasco","year":"2020"},{"key":"e_1_2_9_30_1","volume-title":"Memorias unidad vigilancia epidemiol\u00f3gica","author":"Gobierno Vasco","year":"2021"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13085"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00677-12"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02293-10"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268817000796"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02180-15"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03472-12"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00042-10"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.06.014"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13690-017-0248-y"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12342"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-16-377"},{"issue":"5","key":"e_1_2_9_42_1","first-page":"386","article-title":"Epidemiology of Q fever in Spain (2018","volume":"31","author":"Perez\u2010Arellano J. L.","year":"2018","journal-title":"Revista Espa\u00f1ola de Quimioterapia"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15229"},{"key":"e_1_2_9_44_1","volume-title":"A language and environment for statistical computing","author":"R Core Team","year":"2019"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2016.05.014"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-6-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijheh.2012.12.010"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-69"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2018.09.002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268820002447"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.12871","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/zph.12871","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.12871","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T18:43:06Z","timestamp":1693334586000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.12871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,8]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/zph.12871"],"URL":"https:\/\/doi.org\/10.1111\/zph.12871","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"value":"1863-1959","type":"print"},{"value":"1863-2378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,8]]},"assertion":[{"value":"2021-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}