{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:44:06Z","timestamp":1767167046537,"version":"build-2238731810"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T00:00:00Z","timestamp":1626048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Leptospirosis is a neglected zoonosis with a nearly global distribution. In order to determine the role of bats in the epidemiology of leptospirosis in Nigeria, a total of 231 bats belonging to three families, Pteropodidae (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0117), Molossidae (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0107) and Nycteridae (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a017), roosting in human habitats were screened by PCR and sequencing for the detection of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    species. DNA extracted from the kidneys of bats were subjected to conventional PCR targeting the\n                    <jats:italic>rrs1<\/jats:italic>\n                    ,\n                    <jats:italic>rrs2<\/jats:italic>\n                    ,\n                    <jats:italic>flaB<\/jats:italic>\n                    and\n                    <jats:italic>secY<\/jats:italic>\n                    genes for the detection of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. Overall, 27 out of the 231 (11.7%) of the samples screened were positive for\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. High prevalence (&gt;80%) of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. DNA was detected in\n                    <jats:italic>Chaerophon<\/jats:italic>\n                    and\n                    <jats:italic>Nycteris<\/jats:italic>\n                    bat species captures in an abandoned well located within a human habitation. Sequences generated in this study were highly identical to\n                    <jats:italic>Leptospira borgpetersenii<\/jats:italic>\n                    and\n                    <jats:italic>Leptospira interrogans<\/jats:italic>\n                    and clustered with sequences of pathogenic species in GenBank. The detection of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. was significantly associated (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.001) with the bat species, feeding habit, roosting site and study location. To the best of our knowledge, this is the first molecular detection and characterization of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. in bats from Nigeria. Results show that bats in Nigeria are infected with diverse\n                    <jats:italic>Leptospira<\/jats:italic>\n                    genotypes phylogenetically related to known pathogenic, including zoonotic taxa. Together, these findings reinforce bats\u2019 roles as potential reservoirs of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    spp. and should be considered as a starting point for future comparative studies to improve our understanding of the epidemiology of this bacterial pathogen in Nigeria.\n                  <\/jats:p>","DOI":"10.1111\/zph.12880","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T03:06:31Z","timestamp":1626059191000},"page":"908-916","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular detection and characterization of pathogenic\n                    <i>Leptospira<\/i>\n                    species in bats (Chiroptera) roosting in human habitats in Nigeria, West Africa"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1133-2281","authenticated-orcid":false,"given":"Joshua","family":"Kamani","sequence":"first","affiliation":[{"name":"Bacteriology Parasitology and Virology Department NVRI Vom Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimon","family":"Harrus","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine The Hebrew University of Jerusalem Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reuben A.","family":"Ocholi","sequence":"additional","affiliation":[{"name":"Bacteriology Parasitology and Virology Department NVRI Vom Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene I.","family":"Yague","sequence":"additional","affiliation":[{"name":"Faculty of Ecology and Natural Resource Andres Bello University Santiago de Chile Chile"},{"name":"Servicios de Consultor\u00eda para la Recuperaci\u00f3n y Ordenaci\u00f3n de la Fauna y su Ambiente S.L. 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Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick G.","family":"Nyango","sequence":"additional","affiliation":[{"name":"Biotechnology Division NVRI Vom Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Gonz\u00e1lez\u2010Miguel","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology Institute of Natural Resources and Agrobiology of Salamanca (IRNASA\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Koizumi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3923\/rjvs.2015.21.28"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01043455"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/15321819.2020.1736096"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4102\/ojvr.v84i1.1210"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003899"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddiscovery.2016.48"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171175"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007940"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0349"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1301.060480"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-41.4.753"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002324"},{"key":"e_1_2_9_17_1","first-page":"29","article-title":"Bacteriological survey of leptospirosis in Zaria","volume":"34","author":"Diallo A. 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