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We evaluated the seroprevalence of three zoonotic agents (<jats:italic>Chlamydia felis, Coxiella burnetii<\/jats:italic>and<jats:italic>Toxoplasma gondii<\/jats:italic>) in domestic cats (<jats:italic>Felis catus<\/jats:italic>). Two hundred and ninety\u2010one free\u2010roaming cats were trapped in Murcia municipality (Southeast Spain), and their sera were tested for specific antibodies against<jats:italic>T.\u00a0gondii<\/jats:italic>using a modified agglutination test (MAT), and for<jats:italic>C.\u00a0felis<\/jats:italic>,<jats:italic>C<\/jats:italic>.\u00a0<jats:italic>burnetii<\/jats:italic>and feline immunodeficiency virus (FIV) antibodies with ELISA technique. Pathogen seroprevalence at 95% CI was calculated for each sex and age category (up to and over 12\u00a0months) and compared with a chi\u2010squared test. The role of human population density and urban landscape characteristics on the risk of pathogen exposure in the cat population was explored using generalized linear models. Seropositivity against a single pathogen was found in 60% of the cats, while 19% was seropositive for two or three pathogens. Seroprevalence of<jats:italic>C.\u00a0felis<\/jats:italic>was 8% (CI<jats:sub>95%<\/jats:sub>: 5\u201311), 37% (CI<jats:sub>95%<\/jats:sub>: 31\u201342) for<jats:italic>C.\u00a0burnetii<\/jats:italic>and 42% (CI<jats:sub>95%<\/jats:sub>: 36\u201347) for<jats:italic>T.\u00a0gondii<\/jats:italic>. In addition to these three pathogens, FIV seropositivity was low (1%, CI<jats:sub>95%<\/jats:sub>: \u22120.1 to 2) and adult cats were more likely to be seropositive to<jats:italic>C.\u00a0burnetii<\/jats:italic>than young individuals (OR: 2.3, CI<jats:sub>95%<\/jats:sub>: 1.2\u20134.2). No sex or age class differences in seroprevalence were observed for the rest of the pathogens. Seropositivity was correlated with water surface areas for<jats:italic>C.\u00a0felis,<\/jats:italic>and not with crop areas.<jats:italic>Coxiella burnetii<\/jats:italic>seropositivity was correlated with the percentage of urban areas (continuous with only buildings and discontinuous, that include buildings, parks, and pedestrian and urban green areas), human population size and peri\u2010urban areas with shrubs, and not correlated with other agricultural landscapes (orchards and crop areas). However, the seroprevalence of<jats:italic>T<\/jats:italic>.\u00a0<jats:italic>gondii<\/jats:italic>was only associated with agricultural landscapes such as orchards. The detection of hotspot areas of high pathogen exposure risk is the basis for municipal services to implement surveillance and risk factor control campaigns in specific\u2010risk areas, including (a) efficient health management of urban cat colonies by geographical location, population census and health status monitoring of the components of each cat colony, (b) improvement of hygiene and sanitary conditions at the feeding points of the cat colony and (c) free\u2010roaming cat trapping for health monitoring and, in the long term, to know the evolution of the health status of their populations.<\/jats:p>","DOI":"10.1111\/zph.12919","type":"journal-article","created":{"date-parts":[[2022,2,7]],"date-time":"2022-02-07T13:43:58Z","timestamp":1644241438000},"page":"295-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Urban landscape and infection risk in free\u2010roaming 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Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Serrano","sequence":"additional","affiliation":[{"name":"Wildlife Ecology &amp; Health group (WE&amp;H) and Servei d'Ecopatologia de Fauna Salvatge (SEFaS) Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Domenech","sequence":"additional","affiliation":[{"name":"Department of Food Technology University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Alonso","sequence":"additional","affiliation":[{"name":"Department of Animal Health Regional Campus of International Excellence \"Campus Mare Nostrum\" University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Mart\u00ednez\u2010Carrasco","sequence":"additional","affiliation":[{"name":"Department of Animal Health Regional Campus of 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