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In this study, we analysed the presence of different zoonotic pathogens in 300 clinically healthy goats from 14 zoological gardens in Germany. Rectal and nasal swabs were investigated with a series of cultural and molecular techniques. In addition, vaginal swabs of the 230 female goats were investigated for the presence of <jats:italic>Coxiella burnetii<\/jats:italic> by real\u2010time PCR. Antibodies against <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>burnetii<\/jats:italic> were tested in milk and serum by ELISA. <jats:italic>Campylobacter<\/jats:italic> spp. were found in 22.7%, Shiga\u2010toxigenic <jats:italic>Escherichia coli<\/jats:italic> in 20.0% and <jats:italic>Arcobacter<\/jats:italic> spp. were found in 1.7% of the tested 300 goats after culture from rectal swabs and subsequent PCR. One sample contained an <jats:italic>Escherichia fergusonii<\/jats:italic> isolate with a <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u20101<\/jats:sub>\u2010encoded extended\u2010spectrum beta\u2010lactamase phenotype. Neither <jats:italic>Yersinia<\/jats:italic> spp. nor <jats:italic>Salmonella<\/jats:italic> spp. were found. Nasal swabs of 20.7% of the goats yielded <jats:italic>Staphylococcus aureus<\/jats:italic> including one <jats:italic>mec<\/jats:italic>C\u2010positive methicillin\u2010resistant isolate. Neither <jats:italic>Yersinia<\/jats:italic> spp. nor <jats:italic>Salmonella<\/jats:italic> spp. were found, and none of the 230 vaginal swabs was positive for <jats:italic>C<\/jats:italic>.\u00a0<jats:italic>burnetii<\/jats:italic>. Attempts to detect dermatophytes failed. In conclusion, a possible risk of transmission of zoonotic bacteria from goats in petting zoos to visitors should be considered. Appropriate information and facilities for hand washing and disinfection should be provided in all zoological gardens using goats as contact animals due to the regular presence of zoonotic bacteria in the collection.<\/jats:p>","DOI":"10.1111\/zph.12922","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T04:34:15Z","timestamp":1646109255000},"page":"333-343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Zoonotic bacteria in clinically healthy goats in petting zoo settings of zoological gardens in Germany"],"prefix":"10.1111","volume":"69","author":[{"given":"Jannis","family":"G\u00f6ttling","sequence":"first","affiliation":[{"name":"Zoo Landau in der Pfalz  Landau in der Pfalz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens\u2010Ove","family":"Heckel","sequence":"additional","affiliation":[{"name":"Zoo Landau in der Pfalz  Landau in der Pfalz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Hotzel","sequence":"additional","affiliation":[{"name":"Institute for Bacterial Infections and Zoonoses Friedrich\u2010Loeffler\u2010Institut  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Fruth","sequence":"additional","affiliation":[{"name":"Robert Koch Institute National Reference Centre for Salmonella and other Bacterial Enterics  Wernigerode Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Robert Koch Institute Nosocomial Pathogens and Antibiotic Resistance  Wernigerode Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Henning","sequence":"additional","affiliation":[{"name":"Institute for Bacterial Infections and Zoonoses Friedrich\u2010Loeffler\u2010Institut  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kopp","sequence":"additional","affiliation":[{"name":"IDEXX Vet Med Labor GmbH  Kornwestheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Mertens\u2010Scholz","sequence":"additional","affiliation":[{"name":"Institute for Bacterial Infections and Zoonoses Friedrich\u2010Loeffler\u2010Institut  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Rietschel","sequence":"additional","affiliation":[{"name":"Tier\u00e4rztliches Zentrum f\u00fcr Pferde in Kirchheim Altano GmbH  Kirchheim unter Teck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Pfeffer","sequence":"additional","affiliation":[{"name":"Institute for Animal Hygiene and Veterinary Public Health University Leipzig  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5694\/mja17.00089"},{"key":"e_1_2_9_3_1","first-page":"426","article-title":"Outbreak of shiga toxin\u2010producing Escherichia coli O157 infection associated with a day camp petting zoo \u2013 Pinellas county, Florida, May\u2010June 2007","volume":"58","author":"Alelis K. 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