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Environmental conditions and equine raising or managing practices were recorded in all 72 visited establishments; epidemiological information was obtained from each worker. Microscopic agglutination technique (MAT) was performed with 10 <jats:italic>Leptospira<\/jats:italic> strains for equines and 18 for human sera, that were also studied with IgM indirect immunofluorescence (IgM\u2010IIF). Equine titres \u2265100 were considered positive, and human sera titres \u2265200 suggested probable recent or past infection. Urines were cultured in Ellinghausen\u2013McCullough\u2013Johnson\u2013Harris (EMJH) media; local identification of one obtained isolate with <jats:italic>lipL32<\/jats:italic> PCR, Multiple Locus Variable number tandem repeat Analysis and partial <jats:italic>rrs<\/jats:italic> gene sequencing, were completed at Institut Pasteur, Paris. Estimated reactivity was 61.3% for equines, which was higher than the studied bovine national levels (21%) and mainly observed with Icterohaemorrhagiae serogroup (40.3%), Sejroe, Canicola, Pomona or Ballum. Aged animals from slaughterhouses and cattle farms were the most frequently positive. Multiple regression analysis confirmed a significant association between seropositivity and equine age. Only one positive culture could be fully studied, and confirmed to be <jats:italic>Leptospira interrogans<\/jats:italic> serogroup Canicola; it was added to the MAT antigen panel and revealed fairly frequent reaction with equine and human sera. Three workers (2%) showed titres\u00a0=\u00a0200 with Icterohaemorrhagiae or Canicola serogroups, without recent clinical manifestations. Their attended equines reacted with the same serogroups, suggesting common source infections or infection transmitted by equines. Three other humans yielded titres\u00a0=\u00a0100, and none of the 150 showed an IgM\u2010IIF\u2010positive result. Equines seem not to be an important origin of regional human leptospirosis, except perhaps during acute animal infection. More culture work is required to study intensity and lapses of leptospiruria, as well as to further identify circulating strains.<\/jats:p>","DOI":"10.1111\/zph.12942","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T07:06:23Z","timestamp":1648537583000},"page":"526-536","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Seroprevalence of anti\u2010<i>Leptospira<\/i> antibodies in equines and associated workers\u2014Isolation of <i>Leptospira interrogans<\/i> serogroup Canicola from equine urine"],"prefix":"10.1111","volume":"69","author":[{"given":"Paulina","family":"Meny","sequence":"first","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Iglesias","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jair","family":"Quintero","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"R\u00edos","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Ashfield","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Mosca","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laureana","family":"De Brun","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"De Vries","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Varela","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3379-1032","authenticated-orcid":false,"given":"Felipe","family":"Schelotto","sequence":"additional","affiliation":[{"name":"Bacteriology and Virology Department Hygiene Institute, Medicine Faculty Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1473\u20103099(03)00830\u20102"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19\u20100228"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10111952"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100\u2010736X2011000200009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.07.038"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101552"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13069"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700114"},{"key":"e_1_2_9_10_1","first-page":"109","volume-title":"Leptospira and leptospirosis, current topics in microbiology and immunology","author":"Ellis W. 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