{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:07:58Z","timestamp":1790968078047,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["119O005"],"award-info":[{"award-number":["119O005"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protozoan <jats:italic>Dientamoeba fragilis<\/jats:italic> is one of the most common parasites in the digestive system of humans worldwide. The host range and transmission routes of <jats:italic>D.\u00a0fragilis<\/jats:italic>, including the role of animals, are still ambiguous with few reports from non\u2010human primates, sheep, rodents, pigs, a cat and a dog. In this study, we used microscopic and TaqMan qPCR analyses to investigate <jats:italic>D.\u00a0fragilis<\/jats:italic>in 150 faecal samples from pet budgerigars (<jats:italic>Melopsittacus undulatus<\/jats:italic>) in the Central Anatolia Region of Turkey. <jats:italic>Dientamoeba fragilis<\/jats:italic> DNA was detected in 32 samples, resulting in a mean prevalence of 21.3%. In microscopic examination, trophozoites\/cysts of <jats:italic>D.\u00a0fragilis<\/jats:italic> were detected in 13 of 32 qPCR\u2010positive samples. SSU rRNA sequence analyses of the qPCR\u2010positive isolates identified genotype 1 of <jats:italic>D.\u00a0fragilis<\/jats:italic> as predominant in budgerigars. Phylogenetic analyses of the SSU rRNA gene region clustered <jats:italic>D.\u00a0fragilis<\/jats:italic> genotypes, as well as other trichomonads, in separate monophyletic clusters with bootstrap values \u226579.0. Our study provides the first evidence for the natural host status of pet budgerigars for <jats:italic>D.\u00a0fragilis<\/jats:italic>and contributes to the knowledge of the epidemiology of this parasite. The high prevalence of genotype 1 of <jats:italic>D.\u00a0fragilis<\/jats:italic> suggests that pet budgerigars are suitable reservoirs for zoonotic transmission. Our findings contribute to an increased awareness and knowledge of <jats:italic>D.\u00a0fragilis<\/jats:italic> infections in the context of a one\u2010health approach.<\/jats:p>","DOI":"10.1111\/zph.12949","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T10:00:05Z","timestamp":1650880805000},"page":"572-578","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["First report and genotyping of <i>Dientamoeba fragilis<\/i> in pet budgerigars (<i>Melopsittacus undulatus<\/i>), with zoonotic importance"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5260-3450","authenticated-orcid":false,"given":"Gamze","family":"Yetismis","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9868-0363","authenticated-orcid":false,"given":"Alparslan","family":"Yildirim","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2072-8165","authenticated-orcid":false,"given":"Didem","family":"Pekmezci","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Veterinary Medicine Ondokuz Mayis University  Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6951-0901","authenticated-orcid":false,"given":"Onder","family":"Duzlu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2695-7102","authenticated-orcid":false,"given":"Arif","family":"Ciloglu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6143-3630","authenticated-orcid":false,"given":"Zuhal","family":"Onder","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0492-9840","authenticated-orcid":false,"given":"Emrah","family":"Simsek","sequence":"additional","affiliation":[{"name":"Preclinical Science, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuri","family":"Ercan","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture Kirsehir Ahi Evran University  Kirsehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7791-1959","authenticated-orcid":false,"given":"Gokmen\u00a0Zafer","family":"Pekmezci","sequence":"additional","affiliation":[{"name":"Preclinical Science, Faculty of Veterinary Medicine Ondokuz Mayis University  Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1614-0756","authenticated-orcid":false,"given":"Abdullah","family":"Inci","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Erciyes University  Kayseri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1411.080683"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.2.1.14755"},{"key":"e_1_2_9_4_1","volume-title":"Clinical Parasitology","author":"Beaver J. 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