{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:08:00Z","timestamp":1790968080295,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Dientamoeba fragilis<\/jats:italic> is a flagellated protozoan with amoeba\u2010like morphology that inhabits the human gastrointestinal tract. It is endemic in a vast geography around the world, including developed countries. There are limited studies on non\u2010human hosts of the parasite, and suitable hosts have not been clarified. The parasite has been detected in non\u2010human primates, pigs, cats, dogs and rats. There is no study in the literature investigating and detecting the presence of this parasite in cattle. In this study, stool samples taken from 163 different cattle and calves from 11 different farms between March 2017 and May 2022 were examined for the detection of <jats:italic>D. fragilis<\/jats:italic> via PCR. Trichrome staining was performed on all PCR\u2010positive samples. The isolates with the expected amplicon size were sequenced using the 18S ribosomal RNA region, and their genotypes were determined by BLAST analysis. Sequences were analysed with the most similar and reference sequences in the literature, forming a phylogenetic tree. We detected <jats:italic>D. fragilis<\/jats:italic> in 31 (19.01%) of the 163 stool samples. <jats:italic>D. fragilis<\/jats:italic> cysts\/trophozoites were detected by trichrome staining method in six of 31 samples. All PCR products selected for molecular analysis from positive samples had the same nucleotide sequence. As a result of BLAST analysis, all sequences were determined to belong to <jats:italic>D. fragilis<\/jats:italic> genotype 1. This study determined for the first time that cattle are suitable hosts for <jats:italic>D. fragilis<\/jats:italic>. Furthermore, the parasite subtype we detected belongs to genotype 1, which is the most common type in humans, suggesting that the parasite may have a zoonotic character. Our result is important in terms of the epidemiology of the parasite, as the mode of transmission is controversial, and available data on its suitable hosts are limited.<\/jats:p>","DOI":"10.1111\/zph.12986","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T07:52:23Z","timestamp":1657180343000},"page":"897-903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["First detection and molecular characterization of <i>Dientamoeba fragilis<\/i> in cattle"],"prefix":"10.1111","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8525-6280","authenticated-orcid":false,"given":"\u0130brahim","family":"Yildiz","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Parasitology Adnan Menderes University  Aydin Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Erdem Aynur","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Parasitology Adnan Menderes University  Aydin Turkey"},{"name":"Recombinant DNA and Recombinant Protein Application and Research Center Adnan Menderes University  Aydin Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1893\u20101895.1992"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2009.02894.x"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1805.111093"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.07.025"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2020.102227"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010015\u20100714\u20108"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558\u20105646.1985.tb00420.x"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469\u20100691.2003.00504.x"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2007.09.001"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1645\/13\u2010356.1"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2008.02002.x"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.02877\u201013"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2018.07.008"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.06.003"},{"issue":"1","key":"e_1_2_13_16_1","first-page":"134","article-title":"Ectoparasites and endoparasites of peridomestic house\u2010rats in ile\u2010ife, Nigeria and implication on human health","volume":"9","author":"Ogunniyi T.","year":"2014","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010013\u20101880\u20102"},{"issue":"1","key":"e_1_2_13_18_1","first-page":"113","article-title":"Detection of Dientamoeba fragilis among diarrheal patients referred to Tabriz health care centers by nested PCR","volume":"30","author":"Sarafraz S.","year":"2013","journal-title":"Tropical Biomedicine"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009779"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166\u20106851(95)02516\u20102"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.5578\/mb.66468"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00076\u201015"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.43.6.2718\u20102723.2005"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.10.002"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.10.013"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.05.003"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404206101"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2007.05.006"},{"issue":"4","key":"e_1_2_13_30_1","first-page":"293","article-title":"Dientamoeba fragilis: The unflagellated human flagellate","volume":"56","author":"Windsor J. 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