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The aims of this cross\u2010sectional study were to estimate the prevalence of HEV infections in pigs and humans from different regions of the country, to identify risk factors for increasing anti\u2010HEV IgG prevalence and to characterize HEV strains. The presence of anti\u2010HEV antibodies was assessed by commercial ELISA in serum samples from the general population, farm and slaughterhouse employees, as well as pigs sampled in the three regions of Cuba from February to September 2016. Overall, individuals with occupational exposure to swine or swine products (70\/248, 28.2%) were 4 times more likely to be seropositive compared to the general population (25\/285, 8.7%; OR: 4.18; <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001). Within the risk group, risk factors included age, number of years working in a professional activity with direct exposure to swine, geographic region and distance between residence and closest professional swine setting, while wearing gloves had a protective effect. Prevalence of total anti\u2010HEV antibodies in swine was 88.2% (165\/187) and HEV RNA was detected by real\u2010time RT\u2010PCR in 9.2% (16\/173) swine stools. All HEV strains sequenced clustered within genotype 3. Some strains clearly belonged to subtype 3a, while another group of strains was related with subtypes 3b and 3\u00a0k but partial HEV sequences did not allow unequivocal subtype assignment. These findings suggest that the high HEV exposure in Cuban individuals with swine\u2010related occupations could be due to enzootic HEV in certain regions, direct contact with infectious animals or their products as well as environmental contamination.<\/jats:p>","DOI":"10.1111\/zph.13000","type":"journal-article","created":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T04:49:40Z","timestamp":1663390180000},"page":"58-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hepatitis E virus in Cuba: A cross\u2010sectional serological and virological study in pigs and people occupationally exposed to pigs"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9807-2242","authenticated-orcid":false,"given":"Maria Caridad","family":"Montalvo Villalba","sequence":"first","affiliation":[{"name":"Virology Department Institute of Tropical Medicine Pedro Kouri (IPK)  Havana 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