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To determine whether EPEC isolated from healthy food\u2010producing animals possesses the same virulence gene repertoire as EPEC isolated from human with diarrhoea, we compared six typical EPEC (tEPEC) and 20 atypical EPEC (aEPEC) from humans with diarrhoea and 42 aEPEC from healthy animals (swine, sheep and buffaloes), using pulsed\u2010field gel electrophoresis (PFGE), virulence markers, serotyping and subtyping of <jats:italic>eae<\/jats:italic> and <jats:italic>tir<\/jats:italic> genes. We found that human and animal isolates shared virulence genes, including <jats:italic>nle<\/jats:italic>B, <jats:italic>nle<\/jats:italic>E and <jats:italic>nle<\/jats:italic>F, which were associated with human diarrhoea. Serogroups and serotypes identified in isolates of food\u2010producing animals such as O26:H11, O128:H2, O76:H7, O103, O108, O111 and O145, have previously been implicated in human disease. The subtypes <jats:italic>eae<\/jats:italic> and <jats:italic>tir<\/jats:italic> were also shared between human and animal isolates, being <jats:italic>eae<\/jats:italic>\u2010\u03b31 and <jats:italic>eae<\/jats:italic>\u2010\u03b21 the most prevalent in both groups, while the most common <jats:italic>tir<\/jats:italic> subtypes were \u03b1 and \u03b2. Despite PFGE analysis demonstrating that EPEC strains are heterogeneous and there was no prevalent clone identified, EPEC isolated from humans and food\u2010producing animals shared some characteristics, such as virulence genes associated with human diarrhoea, indicating that food\u2010producing animals could play a role as reservoirs for those genes.<\/jats:p>","DOI":"10.1111\/zph.13007","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T11:10:13Z","timestamp":1668510613000},"page":"117-124","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular analysis of enteropathogenic <i>Escherichia coli<\/i> (EPEC) isolates from healthy food\u2010producing animals and humans with diarrhoea"],"prefix":"10.1111","volume":"70","author":[{"given":"Livia Gerbasi","family":"Beraldo","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, Faculty of Agricultural and 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