{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:40:31Z","timestamp":1792654831203,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["6682"],"award-info":[{"award-number":["6682"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fleas and ticks represent the two main groups of ectoparasites that infest companion animals. In particular, the flea <jats:italic>Ctenocephalides felis felis<\/jats:italic> and several members of the <jats:italic>Rhipicephalus sanguineus<\/jats:italic> complex are the main vectors of a wide range of pathogens on the American continent. They are competent vectors for several members of the genus <jats:italic>Rickettsia<\/jats:italic>, which encompass at least 15 pathogenic obligate intracellular bacteria that colonize the endothelial cells of vertebrates. In Mexico, 10 species of <jats:italic>Rickettsia<\/jats:italic> belonging to three groups have been detected in six species of ectoparasites from dogs in 9 of the 32 states of the country. However, in some larger regions of the country, active epidemiological surveillance has not been carried out. For this reason, the aim of this study was to identify the presence of members of the genus <jats:italic>Rickettsia<\/jats:italic> in fleas and ticks of dogs and cats in the state of Puebla, Mexico. A cross\u2010sectional study was carried out to collect ectoparasites of dogs and cats during August to November 2019. Samples were fixed in 70% ethanol and examined to identify the presence of <jats:italic>Rickettsia<\/jats:italic> DNA by the amplification and sequencing of specific fragments of the <jats:italic>gltA<\/jats:italic> and <jats:italic>ompB<\/jats:italic> genes using conventional PCR. The recovered sequences were compared with those deposited in GenBank, and phylogenetic analyses were carried out to identify the position of the pathogens detected with respect to the valid species previously reported worldwide. Additionally, ecological parameters of the ectoparasite infestations were also calculated. We recovered 196 ectoparasites belonging to two species, 33 <jats:italic>C. felis felis<\/jats:italic> and 163 <jats:italic>R. sanguineus<\/jats:italic> s.l. (<jats:italic>Rhipicephalus linnaei<\/jats:italic>), parasitizing 46 hosts (42 dogs and 4 cats) in 11 localities of the state of Puebla. We detected the presence of <jats:italic>Rickettsia felis<\/jats:italic> in three pools of <jats:italic>C. felis felis,<\/jats:italic> and five from <jats:italic>R. sanguineus<\/jats:italic> s.l. Our work provides the first record of <jats:italic>R. felis<\/jats:italic> in hard ticks of Mexico and Central America, with new collection localities for this pathogen in central Mexico.<\/jats:p>","DOI":"10.1111\/zph.13011","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T15:04:38Z","timestamp":1669043078000},"page":"176-183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular detection of <i>Rickettsia felis<\/i> in fleas and ticks collected from dogs and cats of Puebla, Mexico"],"prefix":"10.1111","volume":"70","author":[{"given":"Beatriz","family":"Salceda\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Laboratorio de Entomolog\u00eda Instituto de Diagn\u00f3stico y Referencia Epidemiol\u00f3gicos, Secretar\u00eda de Salud  Ciudad de M\u00e9xico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia M.","family":"Gasca\u2010Zarate","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias regi\u00f3n Tuxpan Universidad Veracruzana  Tuxpan de Rodr\u00edguez Cano, Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Jim\u00e9nez\u2010Soto","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias regi\u00f3n Tuxpan Universidad Veracruzana  Tuxpan de Rodr\u00edguez Cano, Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefania","family":"Grostieta","sequence":"additional","affiliation":[{"name":"Centro de Medicina Tropical, Unidad de Investigaci\u00f3n en Medicina Experimental, Facultad de Medicina Universidad Nacional Aut\u00f3noma de M\u00e9xico  Ciudad de M\u00e9xico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia G.","family":"L\u00f3pez\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Laboratorio Estatal de Salud P\u00fablica del estado de Puebla Secretar\u00eda de Salud del estado de Puebla  Puebla Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J.","family":"Soto\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda Instituto de Diagn\u00f3stico y Referencia Epidemiol\u00f3gicos, Secretar\u00eda de Salud  Ciudad de M\u00e9xico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1.","family":"Lammoglia\u2010Villag\u00f3mez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias regi\u00f3n Tuxpan Universidad Veracruzana  Tuxpan de Rodr\u00edguez Cano, Veracruz 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Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edelmira","family":"J\u00e1come\u2010Sosa","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias regi\u00f3n Tuxpan Universidad Veracruzana  Tuxpan de Rodr\u00edguez Cano, Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos D.","family":"P\u00e9rez\u2010Br\u00edgido","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias regi\u00f3n Tuxpan Universidad Veracruzana  Tuxpan de Rodr\u00edguez Cano, Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo G.","family":"Ballados\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Rancho Torre\u00f3n del Molino Universidad Veracruzana  Veracruz 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