{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:26:11Z","timestamp":1791905171189,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In August 2021, the Nebraska Department of Health and Human Services was notified by a local public health department of a cluster of two Lyme disease cases in patients with local exposure to wooded areas in a county located in their jurisdiction. Epidemiological investigations revealed that the two patients had similar symptom onset dates and had likely exposure to ticks at wooded sites located directly adjacent to one another. Two environmental investigations were completed in October 2021 and consisted of tick surveys at the patients' reported sites of tick exposure. 12 ticks were collected across the two surveys and identified the black\u2010legged tick (<jats:italic>Ixodes scapularis<\/jats:italic>). During subsequent testing of the collected ticks, spirochete bacteria were isolated, cultured and confirmed as <jats:italic>Borrelia burgdorferi<\/jats:italic> sensu stricto by PCR. In total, 7 of 12 (58.3%) <jats:italic>I. scapularis<\/jats:italic> ticks tested positive for <jats:italic>B. burgdorferi<\/jats:italic> s.s. The results of this study document the fourth known established population of <jats:italic>I. scapularis<\/jats:italic> in Nebraska and confirms the first detection of <jats:italic>B. burgdorferi<\/jats:italic> s.s. in field collected ticks from Nebraska. The epidemiological and environmental investigation data provide the first evidence for local Lyme disease transmission occurring within Nebraska. These findings highlight the need for continued surveillance of <jats:italic>I. scapularis<\/jats:italic> and its associated pathogens in Nebraska to further characterize human risk and monitor emergence into other areas of the state.<\/jats:p>","DOI":"10.1111\/zph.13031","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T08:08:23Z","timestamp":1676362103000},"page":"361-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of <scp><i>Borrelia burgdorferi<\/i><\/scp> infected <scp><i>Ixodes scapularis<\/i><\/scp> (Acari: Ixodidae) associated with local Lyme disease transmission in Nebraska, <scp>USA<\/scp>, 2021"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9951-1577","authenticated-orcid":false,"given":"Jeff","family":"Hamik","sequence":"first","affiliation":[{"name":"Nebraska Department of Health and Human Services  Lincoln Nebraska USA"},{"name":"University of Nebraska  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis J.","family":"Bourret","sequence":"additional","affiliation":[{"name":"Creighton University School of Medicine  Omaha Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Halie","family":"Smith","sequence":"additional","affiliation":[{"name":"Nebraska Department of Health and Human Services  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Herman","sequence":"additional","affiliation":[{"name":"Northeast Nebraska Public Health Department  Wayne Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachael","family":"Birn","sequence":"additional","affiliation":[{"name":"Nebraska Department of Health and Human Services  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy","family":"Dawdy","sequence":"additional","affiliation":[{"name":"Nebraska Department of Health and Human Services  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona J.","family":"Zuffante","sequence":"additional","affiliation":[{"name":"Winnebago Public Health Department  Winnebago Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Donahue","sequence":"additional","affiliation":[{"name":"Nebraska Department of Health and Human Services  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Association of Public Health Laboratories. (2021).Suggested reporting language interpretation and guidance regarding Lyme disease serologic test results.https:\/\/www.aphl.org\/aboutAPHL\/publications\/Documents\/ID\u20102021\u2010Lyme\u2010Disease\u2010Serologic\u2010Testing\u2010Reporting.pdf"},{"issue":"4","key":"e_1_2_10_3_1","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour A. 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