{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:25Z","timestamp":1787016085696,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Eastern Uttar Pradesh region of India is known for its endemicity of acute encephalitis syndrome (AES). Decades of research have established that\n                    <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                    , a causative of scrub typhus, is a substantial contributor (&gt;60%) for the AES cases besides other aetiology, but additional factors in the remaining proportion are still unidentified. Rickettsial infections are challenging to diagnose in clinical settings due to overlapping clinical symptoms, the absence of definitive indicators, a low index of suspicion, and the lack of low\u2010cost, rapid diagnostic tools. Hence, the present study was designed to determine the load of rickettsial infections among AES cases. Furthermore, we aim to find out the prevalent rickettsial species in AES cases as well as in the vector population at this location. The study included the whole blood\/cerebrospinal fluid of AES patients and arthropod specimens from rodents. The molecular identification was performed using the 23S\u20105S intergenic spacer region and\n                    <jats:italic>ompB<\/jats:italic>\n                    gene with genomic DNA obtained from studied specimens. We detected 5.34% (62\/1160) of rickettsial infection in AES cases. Among these, phylogenetic analysis confirmed the presence of 54.8%\n                    <jats:italic>Rickettsia conorii<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934) and 16.1% of\n                    <jats:italic>Rickettsia felis<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910), while the rest proportion of the isolates was unidentified at the species level. Furthermore,\n                    <jats:italic>R<\/jats:italic>\n                    .\u2009\n                    <jats:italic>felis<\/jats:italic>\n                    was identified in one CSF sample from AES patients and three flea samples from\n                    <jats:italic>Xenopsylla cheopis<\/jats:italic>\n                    .\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp. was also confirmed in one\n                    <jats:italic>Ornithonyssus bacoti<\/jats:italic>\n                    mite sample. The results of this investigation concluded the presence of spotted fever group\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    spp. among AES identified cases as well as in the mite and flea vectors that infest rodents.\n                  <\/jats:p>","DOI":"10.1111\/zph.13044","type":"journal-article","created":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T02:13:33Z","timestamp":1682129613000},"page":"403-410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular detection of spotted fever group of Rickettsiae in acute encephalitis syndrome cases from eastern Uttar Pradesh region of India"],"prefix":"10.1111","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-5757","authenticated-orcid":false,"given":"Sthita Pragnya","family":"Behera","sequence":"first","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Singh","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirawati","family":"Deval","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7497-0291","authenticated-orcid":false,"given":"Pooja","family":"Bhardwaj","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0172-7423","authenticated-orcid":false,"given":"Kamran","family":"Zaman","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brij Ranjan","family":"Misra","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niraj","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitali","family":"Srivastava","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok Kumar","family":"Pandey","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajaram","family":"Yadav","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asif","family":"Kavathekar","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajni","family":"Kant","sequence":"additional","affiliation":[{"name":"ICMR\u2010Regional Medical Research Centre BRD Medical College Campus  Gorakhpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay P.","family":"Bondre","sequence":"additional","affiliation":[{"name":"ICMR\u2010National Institute of Virology, Encephalitis Group  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","first-page":"101","article-title":"Spotted fevers & typhus fever in Tamil Nadu","volume":"126","author":"Batra H. 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