{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T02:54:35Z","timestamp":1769223275956,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006393","name":"Universidad de Granada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006393","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Morphological and DNA\u2010based complemented approaches were applied for characterization of sympatric populations of <jats:italic>Phlebotomus longicuspis<\/jats:italic> and <jats:italic>Phlebotomus perniciosus<\/jats:italic> in Morocco. Both sand fly species are generally recorded in sympatry in North Africa but on few occasions have been molecularly characterized. The diagnostic confusion of these species has led to errors in their geographical distribution and probably, in the assignment of their role in the transmission of <jats:italic>L. infantum<\/jats:italic>. Sand flies were caught inside households in El Borouj, central Morocco, in 2014\u20132015. For female sand flies, detection of <jats:italic>L. infantum<\/jats:italic> natural infection and blood meal identification were carried out. According to morphological identification, <jats:italic>Phlebotomus longicuspis<\/jats:italic> s.l. (34.7%) was the second most abundant <jats:italic>Phlebotomus<\/jats:italic> species after <jats:italic>P. sergenti<\/jats:italic>, followed by atypical <jats:italic>Phlebotomus perniciosus<\/jats:italic> (7.1%); 11.6% of the male specimens of <jats:italic>P. longicuspis<\/jats:italic> s.l. were identified as <jats:italic>P. longicuspis<\/jats:italic> LCx according to the number of coxite setae. The density of <jats:italic>Larroussius<\/jats:italic> species was very high (31 <jats:italic>Larroussius<\/jats:italic>\/light trap\/night) in the peripheral neighbourhood of Oulad Bouchair (<jats:italic>p<\/jats:italic>\u2009=\u20090.001) where the first case of cutaneous leishmaniasis due to <jats:italic>Leishmania infantum<\/jats:italic> was detected in 2017. Phylogenetic trees based on three independent genes highlighted three well\u2010supported clusters within <jats:italic>P. perniciosus<\/jats:italic> complex that could be interpreted as corresponding to <jats:italic>P. perniciosus<\/jats:italic>, <jats:italic>P. longicuspis<\/jats:italic> s.s. and an undescribed species, all coexisting in sympatry. Some females with typical morphology of <jats:italic>P. longicuspis<\/jats:italic> were genetically homologous to <jats:italic>P. perniciosus.<\/jats:italic> The taxa cannot be differentiated by morphological methods but characterized by a distinctive genetic lineage for which the synapomorphic characters are described. <jats:italic>Leishmania infantum<\/jats:italic> was detected in females of all clusters with a low parasite load. Population genetics will help to assess the threat of the geographical spread of <jats:italic>L. infantum<\/jats:italic> in Morocco by determining the density, abundance and vector role of the species of the <jats:italic>P. perniciosus<\/jats:italic> complex identified correctly.<\/jats:p>","DOI":"10.1111\/zph.13065","type":"journal-article","created":{"date-parts":[[2023,6,20]],"date-time":"2023-06-20T04:10:12Z","timestamp":1687234212000},"page":"555-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Morphology does not allow differentiating the species of the <i>Phlebotomus perniciosus<\/i> complex: Molecular characterization and investigation of their natural infection by <i>Leishmania infantum<\/i> in Morocco"],"prefix":"10.1111","volume":"70","author":[{"given":"Patricia","family":"Gij\u00f3n\u2010Robles","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"G\u00f3mez\u2010Mateos","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evaristo","family":"Corpas\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naima","family":"Abattouy","sequence":"additional","affiliation":[{"name":"Higher Institute of Nursing and Health Techniques  Tanger Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gemma","family":"Merino\u2010Espinosa","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Morillas\u2010M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoriano","family":"Corpas\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6201-0982","authenticated-orcid":false,"given":"Victoriano","family":"D\u00edaz\u2010S\u00e1ez","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Riyad","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Pathology\/Research Team on Immunopathology of Infectious and Systemic Diseases Hassan II University of Casablanca  Casablanca Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3981-9227","authenticated-orcid":false,"given":"Joaquina","family":"Mart\u00edn\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Pharmacy University of Granada  Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20103305\u20107\u2010421"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4081\/gh.2014.28"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2008.07.003"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/36.1.116"},{"issue":"1","key":"e_1_2_12_6_1","first-page":"139","article-title":"Presence of Phlebotomus perniciosus atypical form in Algeria","volume":"11","author":"Benallal K. 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