{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T21:43:53Z","timestamp":1753739033295,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide. In 2018\u20132022, we investigated the presence of HEV RNA in 1233 stool samples collected in the Qinghai\u2010Tibetan Plateau, including humans (16), Tibetan pigs (624), yaks (312), sheep (267), and dogs (14). HEV RNA was only detected in Tibetan pig faecal samples (18.27%, 114\/624). To perform molecular characterization of HEV strains in Tibetan pigs, we obtained 21 complete HEV genome sequences between 2018 and 2022. Sequence comparisons showed that 21 HEV strains from Tibetan pigs shared the mean nucleotide identities with the reference HEV strains ranging between 82.9% and 94.9% and 89.3% and 92.1% similarities with human HEV strains. Phylogenetic analysis confirmed that all HEV strains were genotype 4, closely related to human HEV strains. Sequence recombinant analysis showed five potential recombinant strains identified in this study, of which SWU\/D18\/2018 (GenBank No. MK410044) was recombinant with human and swine HEV strains, located 6509\u20136878\u2009nt from the recombination point. Based on the Bayesian evolutionary trees, we found that most HEV strains diverged later than human HEV (16 Tibetan pig HEV strains diverged later than 1979, and seven human HEV strains diverged earlier than 1979). Therefore, we speculated that the prevalence of HEV 4 in Tibetan pigs possibly originated from humans in the Qinghai\u2010Tibetan Plateau.<\/jats:p>","DOI":"10.1111\/zph.13078","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T05:07:32Z","timestamp":1697000852000},"page":"120-126","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of hepatitis E virus genotype 4 of probable human origin in Tibetan pigs from the Qinghai\u2010Tibetan Plateau, China"],"prefix":"10.1111","volume":"71","author":[{"given":"Xin","family":"Song","sequence":"first","affiliation":[{"name":"College of Animal &amp; Veterinary Sciences Southwest Minzu University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinbo","family":"Wu","sequence":"additional","affiliation":[{"name":"Animal Husbandry Science Institute of ABa Autonomous Prefecture  Hongyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Agricultural and Rural Bureau of Liangshan Yi Autonomous Prefecture of Sichuan Province  Liangshan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Animal Disease Control and Prevention Ganzi Tibetan Autonomous Prefecture  Kangding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal &amp; Veterinary Sciences Southwest Minzu University  Chengdu China"},{"name":"Key Laboratory of Ministry of Education and Sichuan Province for Qinghai\u2010Tibetan Plateau Animal Genetic Resource Reservation and Utilization  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3893-906X","authenticated-orcid":false,"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal &amp; Veterinary Sciences Southwest Minzu University  Chengdu China"},{"name":"Key Laboratory of Ministry of Education and Sichuan Province for Qinghai\u2010Tibetan Plateau Animal Genetic Resource Reservation and Utilization  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104687"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02334\u201016"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904848106"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83308\u20100"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.86"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a031724"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12934"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.13924"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ve\/vev003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005695"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005521"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02396"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.12.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13050909"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001778"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.000115"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.197985"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.14.9306\u20109314.2005"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.027714\u20100"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2021.03.038"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msq277"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13010075"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.04.033"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010019\u201004410\u2010x"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.13078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T05:43:45Z","timestamp":1705815825000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.13078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,10]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/zph.13078"],"URL":"https:\/\/doi.org\/10.1111\/zph.13078","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"type":"print","value":"1863-1959"},{"type":"electronic","value":"1863-2378"}],"subject":[],"published":{"date-parts":[[2023,10,10]]},"assertion":[{"value":"2022-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}