{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T16:13:35Z","timestamp":1785600815623,"version":"3.56.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000277","name":"Department for Environment, Food and Rural Affairs, UK Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Consumption of pork and pork products is a major source of human infection with <jats:italic>Salmonella<\/jats:italic>. <jats:italic>Salmonella<\/jats:italic> is typically subclinical in pigs, making it difficult to identify infected pigs. Therefore, effective surveillance of <jats:italic>Salmonella<\/jats:italic> in pigs critically relies on good knowledge on how well the diagnostic tests used perform. A test that has been used in several countries for <jats:italic>Salmonella<\/jats:italic> monitoring is serological testing of meat juice using an ELISA (MJ ELISA) to detect antibodies against <jats:italic>Salmonella<\/jats:italic>. This MJ ELISA data could be used to estimate infection prevalence and trends. However, as the MJ ELISA output is a sample\u2010to\u2010positive (S\/P) ratio, which is a continuous outcome rather than a binary (positive\/negative) result, the interpretation of this data depends upon a chosen cut\u2010off.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To apply Bayesian latent class models (BLCMs) to estimate diagnostic accuracy of the MJ ELISA test values in the absence of a gold standard without needing to apply a cut\u2010off.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>BLCMs were fitted to data from a UK abattoir survey carried out in 2006 in order to estimate the diagnostic accuracy of MJ ELISA with respect to the prevalence of active <jats:italic>Salmonella<\/jats:italic> infection. This survey consisted of a MJ ELISA applied in parallel with the bacteriological testing of caecal contents, carcass swabs and lymph nodes (<jats:italic>n<\/jats:italic>\u2009=\u2009625). A BLCM was also fitted to the same data but with dichotomisation of the MJ ELISA results, in order to compare with the model using continuous outcomes. Estimates were obtained for sensitivity and specificity of the ELISA over a range of S\/P values and for the bacteriological tests and were found to be similar between the models using continuous and dichotomous ELISA outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The Bayesian method without specifying a cut\u2010off does allow prevalence to be inferred without specifying a cut\u2010off for the ELISA. The study results will be useful for estimating infection prevalence from serological surveillance data.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13109","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T04:14:13Z","timestamp":1704428053000},"page":"369-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bayesian evaluation of meat juice <scp>ELISA<\/scp> for detecting <i>Salmonella<\/i> in slaughtered pigs without specifying a cut\u2010off"],"prefix":"10.1111","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3009-4672","authenticated-orcid":false,"given":"Mark","family":"Arnold","sequence":"first","affiliation":[{"name":"Animal and Plant Health Agency (APHA)  Loughborough UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6871-4673","authenticated-orcid":false,"given":"Richard Piers","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Epidemiological Sciences APHA  Addlestone UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Martelli","sequence":"additional","affiliation":[{"name":"Department of Bacteriology APHA  Addlestone UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Bacteriology APHA  Addlestone UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.579888"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(96)00990-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(97)01267-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.12.2637"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2004.12.005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/1390675"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1198\/108571106X110883"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.2365"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12916"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02192.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","unstructured":"Davies R. 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