{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:10:48Z","timestamp":1789063848254,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The rat lungworm, <jats:italic>Angiostrongylus cantonensis<\/jats:italic>, has recently been found in the city of Valencia, parasitizing rats, <jats:italic>Rattus norvegicus<\/jats:italic> and <jats:italic>Rattus rattus<\/jats:italic>, its natural definitive hosts. This is the first finding of this zoonotic nematode in continental Europe. After informing local and national health authorities, the collection of local terrestrial snails took place with the aim of elucidating their potential role as intermediate hosts of <jats:italic>A. cantonensis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>A total of 145 terrestrial snails, belonging to the species <jats:italic>Cernuella virgata<\/jats:italic>, <jats:italic>Cornu aspersum<\/jats:italic>, <jats:italic>Eobania vermiculata<\/jats:italic>, <jats:italic>Otala punctata<\/jats:italic>, <jats:italic>Pseudotachea splendida<\/jats:italic>, <jats:italic>Rumina decollata<\/jats:italic> and <jats:italic>Theba pisana<\/jats:italic>, were randomly collected between May and December 2022 in public gardens, parks and orchards in six districts of Valencia, in five of which <jats:italic>A. cantonensis<\/jats:italic> had been reported previously in rats. Once collected and identified, the snails were frozen at \u221220\u00b0C. Subsequently, the DNA was isolated and screened by PCR using specific primers targeting the <jats:italic>A. cantonensis<\/jats:italic> COI gene. Seven individual snails, belonging to the species <jats:italic>C. virgata<\/jats:italic>, <jats:italic>C. aspersum<\/jats:italic> and <jats:italic>T. pisana<\/jats:italic>, were positive, for an overall prevalence of 4.8%. The PCR product from one of them was sequenced by Sanger sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The three positive terrestrial snail species are among the edible species that are frequently included in various dishes in Spain. <jats:italic>C. virgata<\/jats:italic> is reported as a previously unrecorded intermediate host and should be added to the list of more than 200 species of terrestrial snails that have been reported worldwide as intermediate hosts of the rat lungworm. Considering that these terrestrial snails may release infective larvae of <jats:italic>A. cantonensis<\/jats:italic> on leafy green vegetables on which they feed and during their handling and preparation for consumption, prophylactic measures to prevent human neuroangiostrongyliasis in Valencia and other regions to which this zoonotic parasite may spread are recommended.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13131","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T05:50:13Z","timestamp":1711777813000},"page":"451-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Local terrestrial snails as natural intermediate hosts of the zoonotic parasite <i>Angiostrongylus cantonensis<\/i> in the new European endemic area of Valencia, Spain"],"prefix":"10.1111","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9508-6390","authenticated-orcid":false,"given":"M\u00e0rius V.","family":"Fuentes","sequence":"first","affiliation":[{"name":"Parasites and Health Research Group, Department of Pharmacy, Pharmaceutical Technology and Parasitology, Faculty of Pharmacy University of Valencia  Burjassot Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4801-528X","authenticated-orcid":false,"given":"Mercedes","family":"Gomez\u2010Samblas","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Parasitology, Institute of Biotechnology University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orly","family":"Richter","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Parasitology, Institute of Biotechnology University of Granada  Granada Spain"},{"name":"Division of Biology and Medicine Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7537-4176","authenticated-orcid":false,"given":"Sandra","family":"S\u00e1ez\u2010Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Parasites and Health Research Group, Department of Pharmacy, Pharmaceutical Technology and Parasitology, Faculty of Pharmacy University of Valencia  Burjassot Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4898-8519","authenticated-orcid":false,"given":"Rub\u00e9n","family":"Bueno\u2010Mar\u00ed","sequence":"additional","affiliation":[{"name":"Parasites and Health Research Group, Department of Pharmacy, Pharmaceutical Technology and Parasitology, Faculty of Pharmacy University of Valencia  Burjassot Valencia Spain"},{"name":"Laboratorios Lok\u00edmica  Paterna Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-1471","authenticated-orcid":false,"given":"Antonio","family":"Osuna","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Parasitology, Institute of Biotechnology University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6712-9461","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Gal\u00e1n\u2010Puchades","sequence":"additional","affiliation":[{"name":"Parasites and Health Research Group, Department of Pharmacy, Pharmaceutical Technology and Parasitology, Faculty of Pharmacy University of Valencia  Burjassot Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"375","volume-title":"Invertebrats no artr\u00f2podes, Hist\u00f2ria Natural dels Pa\u00efsos Catalans","author":"Altaba C. 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