{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:28:29Z","timestamp":1790252909451,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Protozoan and helminth parasitic infections pose significant public health challenges, especially in developing countries with rural populations marked by suboptimal hygiene practices and socio\u2010economic constraints. The parasites are the etiological agents of these infections and have a notably elevated global prevalence. Therefore, this study focuses on estimating the frequency and transmission dynamics of several parasitic species, including\n                      <jats:italic>Blastocystis, Giardia, Cryptosporidium<\/jats:italic>\n                      spp.,\n                      <jats:italic>Entamoeba histolytica, Ascaris lumbricoides, Trichuris trichiura<\/jats:italic>\n                      ,\n                      <jats:italic>Taenia<\/jats:italic>\n                      spp. and hookworms, within a rural community in southwest Colombia with a particular emphasis on the One Health framework, considering environmental and zoonotic transmission potentials.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      This study involved the analysis of 125 samples, encompassing human participants (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200999), their domestic pets (dogs) (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200924) and water sources (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092). Parasite detection was carried out utilizing a combination of microscopy and molecular techniques. Furthermore, the characterization of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      subtypes (STs) was achieved through Oxford Nanopore sequencing of the rRNA\u201018S gene. The investigation also entailed the examination of potential associations between intestinal parasitism and various sociodemographic factors. Results revealed a high frequency of parasitic infections when employing molecular methods, with\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009109\/87%),\n                      <jats:italic>Giardia<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920\/16%),\n                      <jats:italic>Ancylostoma duodenale<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200928\/22%),\n                      <jats:italic>Ancylostoma ceylanicum<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097\/5.6%),\n                      <jats:italic>E. histolytica<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096\/4.8%),\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912\/9.6%) and even\n                      <jats:italic>Taenia<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091\/0.8%) detected.\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. was also identified in water samples. Coinfections were prevalent, with 57% (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200970) of samples exhibiting single\u2010parasite infections and 43% (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200953) showing various degrees of polyparasitism, emphasizing the complexity of transmission dynamics.\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      subtyping, conducted via Oxford Nanopore sequencing, revealed a diversity of subtypes and coexistence patterns, with ST2 being the most prevalent.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This research underscores the importance of using molecular techniques for frequency estimation, particularly emphasizing the relevance of zoonotic transmission in parasitic infections. It highlights the significance of the One Health approach in comprehending the circulation of parasites among animals, humans and environmental sources, thereby directly impacting public health and epidemiological surveillance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/zph.13138","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T20:58:50Z","timestamp":1714510730000},"page":"723-735","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular detection of intestinal parasites in a rural community of Colombia: A one health approach to explore potential environmental\u2013zoonotic transmission"],"prefix":"10.1111","volume":"71","author":[{"given":"Sergio","family":"Casta\u00f1eda","sequence":"first","affiliation":[{"name":"Centro de Investigaciones en Microbiolog\u00eda y Biotecnolog\u00eda\u2010UR (CIMBIUR), Facultad de Ciencias Naturales Universidad del Rosario  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Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rojelio","family":"Mej\u00eda","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Tropical Medicine Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan David","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Microbiolog\u00eda y Biotecnolog\u00eda\u2010UR (CIMBIUR), Facultad de Ciencias Naturales Universidad del Rosario  Bogot\u00e1 Colombia"},{"name":"Molecular Microbiology Laboratory, Department of Pathology, Molecular and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York city New York 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